纳米抗体是一种具有相对分子质量小、稳定性好、可溶性强、抗原结合性能好和免疫原性低等特点的新型抗体。 骆驼体内存在天然的缺失轻链的重链抗体,克隆其可变区可以得到只有重链可变区组成的单域抗体,称为VHH(Variable domain of heavy chain of heavy chain antibody),也称为纳米抗体(nanobody, a single domain antibo...
The specific process is as follows: After obtaining the nanobody sequence library, the nanobody expression library is established by high-throughput primer synthesis and vector construction. Then, the plasmids are transfected into HEK293 cells in shake flasks. Nanobodies are then purified by one-ste...
Dormeshkin D, Shapira M, Dubovik S, et al. Isolation of an escape-resistant SARS-CoV-2 neutralizing nanobody from a novel synthetic nanobody library[J]. Frontiers in Immunology, 2022, 13: 965446. Swindells M B, Porter C T, Couch M, et al. abYsis: integrated antibody sequence and str...
抗体的体外选择允许快速且低成本地获得功能强大的结合剂(binders)。在这里,我们描述了人源化的美洲驼单域抗体的第一个完全合成的噬菌体展示库(NaLi-H1:Nanobody Library Humanized 1)。基于针对细胞内稳定性优化的人源化合成单结构域抗体(hs2dAb, humanized synthetic single domain antibody)支架,高度多样化的文库无...
•VHH抗体是迄今为止发现的具有结合抗原功能的最小分子量抗体片段,分子量为15kD，仅为常规抗体的1/10，其分子高度4.8nm，直径在2.2nm，故被称为纳米抗体(nanobody)•VHH抗体特点：①分子量小，易表达；②高水溶性和稳定性（耐高温、耐极度pH值）；③可识别独特构造的抗原表位 图1.传统抗体与骆驼抗体比较...
Hattab, et al., V(H)H (nanobody) directed against human glycophorin A: a tool for autologous red cell agglutination assays, Anal. Biochem. 438 (2013) 82-89.VHH(nanobody)directed against human glycophorin A:a tool forautologous red cell agglutination assays. Habib I,Smolarek D,Hattab C,...
•VHH抗体是迄今为止发现的具有结合抗原功能的最小分子量抗体片段,分子量为15kD,仅为常规抗体的1/10,其分子高度4.8nm,直径在2.2nm,故被称为纳米抗体(nanobody)•VHH抗体特点:①分子量小,易表达;②高水溶性和稳定性(耐高温、耐极度pH值);③可识别独特构造的抗原表位图1.传统抗体与骆驼抗体比较模型图。骆驼...
Dormeshkin D, Shapira M, Dubovik S, et al. Isolation of an escape-resistant SARS-CoV-2 neutralizing nanobody from a novel synthetic nanobody library[J]. Frontiers in Immunology, 2022, 13: 965446. Swindells M B, Porter C T, Couch M, et al. abYsis: integrated antibody sequence and str...
Mitchell, L. S. & Colwell, L. J. Comparative analysis of nanobody sequence and structure data.Proteins Struct. Funct. Bioinforma.86, 697–706 (2018). ArticleCASGoogle Scholar De Genst, E. et al. Molecular basis for the preferential cleft recognition by dromedary heavy-chain antibodies.Proc...
VHH抗体是迄今为止发现的具有结合抗原功能的最小分子量抗体片段,分子量为15kD,仅为常规抗体的1/10,其分子高度4.8nm,直径在2.2nm,故被称为纳米抗体(nanobody)VHH抗体特点:①分子量小,易表达;②高水溶性和稳定性(耐高温、耐极度pH值);③可识别独特构造的抗原表位图1.传统抗体与骆驼抗体比较模型图。骆驼抗体缺少...