Specific pathogen-free BALB/c female mice (6–8 weeks old) were obtained from the Experimental Animal Center of the Institute of Medical Biology, Chinese Academy of Medical Sciences. All mice used in this study were in a healthy state and were raised in SPF animal facilities of the Institute...
A reference-free alignment strategy based on the XMIPP [26] processing package was used to align selected particles and sort them into self-similar groups of classes. 2.5. Cloning, Expression, and purification of Wild-Type and Mutant Hemagglutinins Influenza virus HA wild-type sequences were ...