Cells of yeasts were cultivated in 50 mL of YEPD medium (1% m/v yeast extract, 2% m/v peptone, 2% m/v d-glucose) at 150 r.p.m (Incubator Shaker, model SL 222, SOLAB Equipamentos para Laboratórios, Piracicaba, SP, Brazil) at 37 °C for 18 h. Soon after the growth,...
Target cells were detached and approximately 1.5 × 104 cells in 50 µL complete DMEM were added per well, prior to incubation in a humidified cell-culture incubator for 48 h at 37 °C in 5% CO2. Equal volumes of Bright-Glo™ reagent and Phosphate Buffered Saline (PBS) were mixed ...
Needham, Brittany D., and M. Stephen Trent. “Fortifying the barrier: the impact of lipid A remodelling on bacterial pathogenesis.” Nature Reviews Microbiology 11.7 (2013): 467-481. Orenstein, Robert, and Edward S. Wong. “Urinary tract infections in adults.” American family physician 59.5 ...
coli and the serotypes of C. jejuni present in broilers from selected small retail processors in six counties in Trinidad. Design/methodology/approach – Samples were plated on blood-free Campylobacter CCDA, incubated at 42°C in 8-10 per cent CO2 in a CO2 incubator and isolates were bio...
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Animal cell culture is a type of biotechnological technique where animal cells are artificially grown in a favorable environment.
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The optimum and maximum temperatures of growth in 1 : 10 diluted broth, as determined in a temperature gradient incubator were between 20 and 32, and between 29 and 34°C with an average of 26 and 31°C, respectively. Based on these results a very simple nutrient-tolerance test was ...
The Bacillus subtilis (ATCC 6633) inoculum was prepared into a 250 mL flask containing 100 mL of Luria-Bertani (LB) media with peptone (5 g), meat extract (3 g), and NaCl (5 g) and incubated at 30 °C for 24 h using a shaking incubator (150× rpm). The prepared inocula ...
Next, 10-mL aliquots of pseudoviruses (105 RLU) were added to each well, and the mixtures were incubated for 1 h in a CO2 incubator at 37 °C under an atmosphere with 5% CO2. After incubation, an aliquot of each mixture was added to a monolayer of cells. As a negative control, ...