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After 3 washes with PBS, cells were incubated at room temperature for 1 h with the secondary antibody Goat Anti-Mouse IgG H&L- Alexa Fluor® 488 (1:250, Abcam, ab150113) and Hoechst 33342 (1 μg/mL, Invitrogen, H1399). Cells were imaged at 40× magnification, using the IN ...
Ozone is gaseous at room temperature, colorless and grassy at low concentration, and soluble in water. It is known as one of the strongest oxidants. The chemical properties of ozone are more active and have strong oxidation ability. When met with bacteria and harmful che...
All cells were washed twice with PBS, fixed for 1 h in formalin at room temperature and then washed three times for 5 min/wash with PBS. Feulgen DNA staining was accomplished according to Pearse [26] without other counterstaining. After rinsing, dehydration in graded ethanol, the preparations...
Ozone is gaseous at room temperature, colorless and grassy at low concentration, and soluble in water. It is known as one of the strongest oxidants. The chemical properties of ozone are more active and have strong oxidation ability. When met with bacteria and harmful chemicals ...
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This intervention would allow the homogeneous atomization of the deacidifying agent in alcoholic solvents to be safe and effective for the work as a whole. Keywords: pastel painting; contemporary art; spolvero paper; deacidification; nanostructured calcium hydroxide Academic Editors: Andrea Macchia, ...
17 A post hoc analysis investigated the rates of asymptomatic and symptomatic intracranial hemorrhage using the European Cooperative Acute Stroke Study III (ECASS III), ECASS II, the Safe Implementation of Thrombolysis in Stroke–Monitoring Study (SITS-MOST), and the National Institute of Neurological...
Then the sections were washed with distilled water and stained with FJC (0.001% in distilled water) for 30 min at room temperature in the dark, while undergoing gentle shaking. After staining, the sections were washed 3 times with distilled water for 1 min each before being air dried. ...