结论 TPM1基因表达的抑制可能参与鼻咽癌的发生。%Objective To investigate the relation between TPM1 gene expression and nasopharyngeal carcinoma (NPC). Methods TPM1 expression was examined in three NPC cell lines (CNE1, CNE2 and 58F) and one nasopharyngeal epithelial cell line (NP69) by real ...
有了每一个gene 的 TPM_g ,就可以算全部G个gene的TPM的平均值: \bar{TPM} = \frac{\sum_{g=1}^{G}{TPM_g}}{G} = \frac{10^6}{G} Sure, TPM的平均值是一个只依赖于基因总数目G的量,符合第一性原理。 接下来是 RPKM,reads per kilobase per million reads, RPKM_g = \frac{r_g *...
Dysregulated microglia, on the other hand, are associated with neurodegenerative diseases, yet the mechanisms underlying pro-inflammatory gene expression in microglia are incompletely understood. Methods:We investigated the role of the actin-associated protein tropomyosin 1 (TPM1) in regulating pro-...
并且整体文库更大的样本也会获得更多的reads,例如A样本测了1百万条reads,而B样本只测了一万条reads,显然单纯的将gene count作为表达量将会造成大量的基因在A样本中较B样本上调,这显然不是由于生物学因素造成的,而是由文库大小引入的。因此"gene count→gene expression"的转换需要充分地考虑到基因长度、文库大小等因素...
TPM1 Isoform 1 is expressed in adult and fetal skeletal muscle and cardiac tissues, with higher expression levels in the cardiac tissues, whereas Isoform 10 is expressed in adult and fetal cardiac tissues, but not in skeletal muscle. Mutations in the TPM1 gene are linked to type 3 familial ...
并且整体文库更大的样本也会获得更多的reads,例如A样本测了1百万条reads,而B样本只测了一万条reads,显然单纯的将gene count作为表达量将会造成大量的基因在A样本中较B样本上调,这显然不是由于生物学因素造成的,而是由文库大小引入的。因此"gene count→gene expression"的转换需要充分地考虑到基因长度、文库大小等...
bulk and single-cell RNA-seq expression units, count normalization, formula, examples in Python, gene quantification, batch effects, and between-sample and within-sample normalization
由此表明,小分子化合物可诱导心脏成纤维细胞逐渐丧失多能性,定向分化为心肌细胞,并具有较高的诱导效率.2.Tpm1是正向调控CFs向心肌细胞分化的重要基因为了明确在诱导过程中起重要调控作用的关键基因,首先提取诱导组和对照组RNA进行转录组序列分析.根据测序结果对表达差异的基因进行GO富集分析及KEGG通路分析.利用RTPCR技术...
MicroRNAs (miRNAs)2 are a class of naturally occurring small noncoding RNAs that regulate gene expression by targeting mRNAs for translational repression or cleavage (1, 2). Like protein-coding mRNAs, miRNAs are transcribed as long primary transcripts in the nucleus. However, unlike protein-coding...
最后你下载的TCGA FPKM数据转换TPM就不会懵逼了吧 参考文献:https://academic.oup.com/bioinformatics/article/26/4/493/243395/RNA-Seq-gene-expression-estimation-with-read