In contrast, in NEMO-A323P cells a rapid and exacerbated cell death after treatment with TNF/zVAD was detected (Figure 1c). This cell death was blocked by the RIPK1 kinase inhibitor Nec-1 (Figure 1c).29 In line with a protective role of NEMO, NEMO- KO cells became more sensitive ...
zVAD-induced necroptosis in L929 cells depends on autocrine production of TNFα mediated by the PKC-MAPKs-AP-1 pathway. Cell Death Differ 2011; 18: 26–37. 53. Kearney CJ, Cullen SP, Tynan Ga, Henry CM, Clancy D, Lavelle EC et al. Necroptosis suppresses inflammation via termination of ...
Barazzone. TNF- induced enterocyte apoptosis and detachment in mice: induction of caspases and prevention by a caspase inhibitor, ZVAD-fmk. Lab. Invest. 79: 495-500, 1999.Piguet PF,Vesin C,Donati Y,Barazzone C. TNF-induced enterocyte apoptosis and detachment in mice:induction of caspases ...
not been fully elucidated, and probably depend on the particular metabolic status of the cell examined (i.e., anerobic glycolysis in mostin vitrocell linesversusoxidative metabolism of neuronal cells, see Welsbyet al.12for review). In any instances, and independently of the fine molecular events...
3C). Such an early boost in RIPK1 activity was not observed at the receptor complex upon stimulation of cells with the necroptotic trigger TNF + zVAD-fmk (Fig. 3D), but was instead detected, at around the same time, in a pool of cytosolic RIPK1 (Fig. 3E). In these experiments...
Sensitisation to TNF-induced apoptosis was prevented by the broad caspase inhibitor zVAD-fmk, whereas overexpression of Bcl-2 had no effect. Mithramycin also potentiated cell death induced by Fas agonistic antibodies. In contrast, mithramycin reduced the percentage of cells undergoing apoptosis due to ...