PROBLEM TO BE SOLVED: To provide an RNA aptamer molecule having binding ability to human TNF-α.YOSHIDA YOSHIHITO吉田 嘉仁HORII KATSUNORI堀井 克紀FURUICHI MAKIO古市 真木雄WAGA IWAO和賀 巌OMI KAZUYA小見 和也
Biotin-labeled DNA aptamer was captured on the streptavidin chip surface then TNF-α injected into the surface. An Anti-TNF-α antibody injected aptamer-TNF-α pair on the surface for the enhancement step. They reported CV% values in the 3–8% range and concluded that their biosensor was ...
TNF -. Alpha. - binding aptamers and their therapeutic usesThe present invention discloses a TNF -. Alpha. Binding RNA aptamer shown in sequence number 1 or array number 2 and its therapeutic application. No selectionソンチョンキム
Aptamer therapeuticsBiosensorsTumor necrosis factor-alpha (TNF-α) is a pivotal cytokine in the pathogenesis of numerous inflammatory and autoimmune diseases. Precise and sensitive detection of TNF-α is essential for both clinical applications and research endeavors. In the realm of cytokine detection,...
Therefore, the aptamer of the present invention may be used to detect TNF- and provide a therapeutic method for diseases related to the increasing level of TNF-. Compared with other TNF antagonists such as monoclonal antibody and soluble receptor, the present invention has high specificity, high ...
aptameraptamer dimerizationinflammatory diseasesSELEXThe human tumor necrosis factor alpha (hTNF-alpha) is an important pro-inflammatory cytokine which plays critical roles in inflammatory diseases such as rheumatoid arthritis (RA). The anti-TNF-alpha proteins can reduce symptoms of RA. Due to ...
TNF-Alpha-Binding Aptamer, And Therapeutic Use For SameThe present invention relates to a TNF-α-binding RNA aptamer disclosed in SEQ ID NO: 1 or SEQ ID NO: 2, and to the therapeutic use thereof.SUNG-CHUN KIM
RNA aptamers for tumor necrosis factor-alpha (TNF alpha), for which functionality was demonstrated in L929 cells, show only little affinity for the protein in vitro. Detailed investigation of the aptamer-protein interaction by surface plasmon resonance and quartz crystal microbalance analysis revealed ...
Therefore, the aptamer of the present invention may be used to detect TNF- and provide a therapeutic method for diseases related to the increasing level of TNF-. Compared with other TNF antagonists such as monoclonal antibody and soluble receptor, the present invention has high specificity, high ...
TNF-.ALPHA.-BINDING APTAMER, AND THERAPEUTIC USE FOR SAMEDisclosed are a TNF-a-binding RNA aptamer which is disclosed by SEQ ID NO: 1 or SEQ ID NO: 2, and a therapeutic use for same.tnf-a-binding aptamer, and therapeutic use for same...