THP-1细胞诱导分化为巨噬细胞后，可以通过摄取大量脂质转变为泡沫细胞，这也是动脉粥样硬化产生的原因。将...
该细胞系是个用于人制备的人二倍体Cells;培养基中添加TNFα可以加快细胞生长。;细胞传代方法:1:2-1:4传代;2-3天换液1次;细胞生长特性:贴壁生长;细胞形态特性:成纤维细胞样;相关细胞有:J774 A.1Cells、SNU-761Cells、Walker/LLC-WRC256细胞 HT 1080.TCells;细胞背景资料:该细胞源自一名35岁患有纤维肉瘤的...
该细胞表达WNT3和WNT78。TNF alpha抑制该细胞生长。该细胞雌激素受体阴性,但表达5'外显子缺失的雌激素mRNA。 RIN-m5f细胞系;细胞传代方法:1:2-1:3传代;每周换液2-3次;细胞形态特性:详见细胞说明书;细胞生长特性:贴壁;细胞背景资料:胰岛β细胞瘤;雄性;NEDH...
发现与THP-1细胞共培养对人肠上皮样Caco-2细胞单层诱导损伤,涉及LDH释放增加和TER值降低。通过添加抗TNF-α抗体,可以完全阻止从Caco-2细胞单层释放的LDH的增加,并且还可以明显地恢复TER值的降低。此外,依那西普还显着抑制了LDH释放的增加,并且TNF-α处理显着诱导了细胞损伤,提示TNF-α是THP-1细胞分泌的初始因子,...
TNF-alpha release by monocytic THP-1 cells through cross-linking of the extended V-region of the oral streptococcal protein I/II. J. Leukoc. Biol. 67:81-89.Chatenay-Rivauday, C. , Yamodo, I. , Sciotti, M.A. , Troffer-Charlier, N. , Klein, J.P. , and Ogier, J.A. ( 2000...
细胞简称:THP-1KO-TNFR1&TNFR2 产品货号:CGKO-M2196 修饰基因:TNFR1,TNFR2 修饰类型:敲除 转导方法:蛋白电转 抗性基因:— 克隆类型:双敲纯合子 细胞鉴定:PCR,测序 种属来源:人 组织来源:外周血 疾病特征:急性单核细胞白血病 细胞形态:单核细胞样 ...
Role of TNF-alpha and extracellular ATP in THP-1 cell activation following allergen exposure [J]. J Toxicol Sci, 2008, 33(1): 71-83. [20] Ouwehand K, Spiekstra S W, Waaijman T, et al. Technical advance: Langerhans cells derived from a human cell line in a full-thickness skin ...
(Phorbol-12-myristate-13-acetate,PMA)诱导成巨噬细胞,再分别以β2-AR激动剂以及对β2-AR不同选择性的β受体阻滞剂干预巨噬细胞,分别观察β2-AR激动剂以及对β2-AR不同选择性的β受体阻滞剂对巨噬细胞吞噬ox-LDL的能力和吞噬ox-LDL后的细胞存活率,以及释放肿瘤坏死因子-α(Tumor necrosis factor alpha,TNF...
MR, mannose receptor; SRs, scavenger receptors; TLR, Toll-like receptor, TGF-β, transforming growth factor beta; TNF-a, tumor necrosis factor alpha; LPS, lipopolysaccharides; PMA, Phorbol 12-myristate 13-acetate. Similarly, the M1 polarization can be achieved by exposure to IFN-γ and LPS ...
Background Tumor necrosis factor-alpha (TNF-α) is a pro-inflammatory cytokine today identified as a key mediator of several chronic inflammatory diseases. TNF-α, initially synthesized as a membrane-anchored precursor (pro-TNF-α), is processed by prote