Reports on the design of the Oligonucleotide Probe Database (OPD) to address difficulties in relying on literature for information on oligonucleotide hybridization probes and polymerase chain reaction (PCR) pri
oligonucleotide probes (Supplementary Table3) and amplified with Alexa Fluor 488 tyramide at 46 °C for 15 min. After CARD–FISH, the filters were embedded in photographic emulsion (K5, ILFORD) and exposed at 4 °C for 14 days in the dark with silica gel as a drying agent. Develop...
The oligonucleotide probe sequence was designed using Primer 5.0 (Primer-E Ltd., Plymouth, UK) (Supplementary Data 10). The fluorescent probe was synthesized by BGI (China). Fluorescence in situ hybridization was performed using the RNA-FISH Kit (GenePharma, China). Afterwards adhesion slides ...
We designed probes spanning 50 kbp regions using oligonucleotide libraries produced by massively parallel synthesis at the anchors of four hyperconnected 3D cliques: two strain-similar regions, Bcl11b and Ets1, and two diabetes-associated regions, Idd9.2 and Idd6.AM. To corroborate the multi-...
Gong P, Pirooznia M, Guan X, Perkins EJ: Design, validation and annotation of transcriptome-wide oligonucleotide probes for the oligochaete annelid Eisenia fetida. PLoS One. 2010, 5 (12): e14266-10.1371/journal.pone.0014266. Article PubMed Central PubMed Google Scholar Heyland A, Vue Z, ...
The sequence of the probe encompassing the putative ETS site is: CCTCAAGGAAGA GATCACAG. The predicted binding site for ETS is shown italicized in the oligonucleotide sequence. This site corresponds to base positions -227 to -230 in the aligned sequence. The oligonucleotides used to examine ...
The probes are solution-based, biotinylated oligonucleotide sequences that are designed to hybridize and capture the regions intended in the design. Capture probes are significantly longer than PCR primers and therefore can tolerate the presence of several mismatches in the probe binding site without ...
Source Data Full size image We next examined the involvement of TLR9 relative to cGAS–STING signalling in memory using pharmacological manipulations of these pathways. The TLR9 antagonist oligonucleotide ODN2088 significantly impaired CFC, whereas the small drug cGAS–STING inhibitors RU-521 and H-151...
Chalifa-Caspi V, Yanai I, Ophir R, Rosen N, Shmoish M, Benjamin-Rodrig H, Shklar M, Stein TI, Shmueli O, Safran M, Lancet D (2004) GeneAnnot: comprehensive two-way linking between oligonucleotide array probesets and GeneCards genes. Bioinformatics 20(9):1457–1458 ...
Equal loading was verified using a probe for U6 snRNA (data not shown). A file (Additional data file 5) with details of microarray design and data analysis. And a file (Additional data file 6) with a summary of the microRNA microarray data. Oligonucleotide sequences correspond to probes ...