To investigate ribosome stalling upstream of theGag-Polframeshift site, we performed codon-resolved analysis using the Ribo-seq data. Ribosomes showed minor pausing at the P-site UUA (Leu) of the slippery site and a prominent pause at the AGG (Arg) codon 74 nucleotides upstream, consistent w...
Since, as is known in the art, the translation initiation codon is typically 5′-AUG (in transcribed mRNA molecules; 5′-ATG in the corresponding DNA molecule), the translation initiation codon is also referred to as the “AUG codon,” the “start codon” or the “AUG start codon”. A...
This arrangement can be represented as: 5′ORF3′-IGR-5′ORF3′. In a further embodiment, the site for insertion of the exogenous DNA sequence into the IGR is downstream, i.e. 3′, of the stop codon of a first ORF; and, in case the second ORF has an opposite orientation relative ...
Besides, the present inventors have found that the rhtA gene amplification also conferred resistance to homoserine and threonine. The rhtA23 mutation is an A-for-G substitution at position −1 with respect to the ATG start codon (ABSTRACTS of 17thInternational Congress of Biochemistry and Molecula...