PURPOSE:To enhance the dialyzing performance and ultrafiltration speed of a cuprammonium cellulose made membrane by a method wherein either one side or both sides of a membrane is formed as a dense layer of a specified thickness, and the layer directly inside the dense layer is formed as a...
The lysis buffer used for a particular application depends on the target proteins that will be immunoprecipitated, because the location of the protein in the cell (e.g., membrane, cytosol, nucleus) affects the ease of release during ...
The Dialysis Outcomes and Practice Patterns Study (DOPPS) is a prospective, international, observational study of hemodialysis practices and associated outcomes. The primary goal of DOPPS is to improve the understanding of dialysis practices that are associated with better outcomes for patients. The ...
Citation34 The bimolecular structure of the liposome membrane is similar to that of the cell membrane and exhibits good biocompatibility, thus, liposomes are more conducive to drug binding and are considered to be the best drug carrier system thus far.Citation35 Furthermore, the combination of ...
The lipid-free apoA-I that was not incorporated in the lipid protein complexes was removed from the preparation either by dialysis using a dialysis membrane with a molecular weight cut-off of 50,000 or by gel filtration using an Amersham Pharmacia Biotech Superose 6HR column, 10/30 (total ...
7A and B). The absence of cellular toxicity was confirmed by measuring changes in membrane integrity using CellTox Green, a cyanine dye that is excluded from living cells but stains the DNA of dead cells (Supplementary Fig. 5). Indeed, Molidustat did not increase the CellTox Green signal ...
Abraham MR, Selivanov VA, Hodgson DM, Pucar D, Zingman LV, Wieringa B, Dzeja PP, Alekseev AE, Terzic A (2002) Coupling of cell energetics with membrane metabolic sensing. Integrative signaling through creatine kinase phosphotransfer disrupted by M-CK gene knock-out. J Biol Chem 277:24427...
The TRPV1 S1-S4 membrane domain couples chemical ligand binding to the pore domain during channel gating. Here we show that the S1-S4 domain also significantly contributes to thermosensing and couples to heat-activated gating. Evaluation of the isolated human TRPV1 S1-S4 domain by solution ...
Choline is a cellular proliferation marker. The mechanism of uptake of18F-labeled or11C-labeled choline analogues is not fully understood. As choline is a precursor of phosphatidylcholine, a phospholipid constituent of the cell membrane, its uptake, and processing for construction and maintenance of ...
1a). Transport across the IE membrane is mediated by a diverse set of proteins (for example, TGD-1, PPT, TPT, NNT)2, whereas the OE membrane was initially considered to be a permeable sieve that cannot form a barrier for small molecules3. This view was questioned by the finding that ...