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CHO cells stably expressing human S1P1,2,3,4,5were used (Mandala et al.2002). cDNA sequences encoding rodent S1P receptors were cloned from genomic DNA by polymerase chain reaction using the following primers for each respective receptor: 5′-GAACCCGGGTGTCCACTAGCATCCCGG and 5′CCCGAATTCTTAG...
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The Primer3 software was used to design the following primers: Q-forward GAAGTTGAAGCTGCAAGGGCG, and Q-reverse, ATTGCCTCATTTTCGGCGTCGG, GAPDH-forward AACGAGTGGGGATACAGCACCC and GAPDH-reverse CAAACCACTCTCCCCTGTATGCC. The Promega GoTaq® 1-Step RT-qPCR System was followed as per the ...
Aims Syncope is transient loss of consciousness (TLOC) caused by transient cerebral hypoperfusion. Neurally mediated syncope (NMS) is the most common cause. The head-up tilt test (HUTT) is the gold standard for diagnosing and categorisin... G Parker,J Kelly,M Smith,... - 《Archives of...
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Gene encoding 2N4R tau protein was amplified from human whole brain Marathon®-Ready cDNA library (Clontech) using primers 5′-catatggctgagccccgccaggagttcgaagtgatg (forward) and 5′-ctcgagtcacaaaccctgcttggccagggaggcagac (reverse) and cloned into the pET24a + E. coli expression vector...
4180G>C, gene conversion to CYP2D7 in exon 9 *40 NF Not tested 1023C>T, 1661G>C, 1863ins(TTT CGC CCC)2, 2850C>T, 4180G>C *41 DF 1661G>C, 2850C>T, 2988G>A, 4180G>C −1584C, 1661G>C, 2850C>T, 4180G>C Duplication IF Nucleotide changes in bold define the allele....
$$ \begin{array}{ccc} 0: 000 & 4: 100 \\ 1: 001 & 5: 101 \\ 2: 010 & 6: 110 \\ 3: 011 & 7: 111 \\ \end{array} $$ Let us start with the first test pattern {0, 0, 0} and add it to the test set. The initial antirandom test set was empty and first pattern ...
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