试剂 使用量 5 × PrimeScript Buffer 2 μl Random 6 mers (100 μM) *1 2 μl Oligo dT Primer (μ50M) *1 0.5 μl PrimeScript RT Enzyme Mix I 0.5 μl Total RNA X μl RNase Free dH 2 O Up to 10 μl Total 10 μl*2 *1 Random 6 mers 和 Oligo dT Primer 同时使用,可有效地...
制品中含有Real Time PCR反应的适浓度TB Green,是一种2×浓度的Premix Type试剂,进行实验时,PCR反应液的配制十分方便简单。 本制品中还添加了Tli RNaseH(耐热性RNaseH),以cDNA作为模板进行PCR反应时,可以很好地抑制地由于cDNA中残存mRNA对PCR反应造成的阻害作用。 本制品Buffer经过改良,使反应特异性比TB ...
PCR Reverse Primer(10 μM) 0.4 μl 0.2 μM* 1 DNA 模板(<100 ng)* 2 2.0 μl dH2O(灭菌蒸馏水) 7.2 μl Total 20.0 μl *1 通常引物终浓度为0.2 μM可以得到较好结果。反应性能较差时,可以在0.1～1.0 μM 范围内调整引物浓度。 *2 DNA 模板的添加量通常在100 ng 以下。因不同种类的...
与RealTimePCR用最适Buffer组合,可以有效抑制非特异性的PCR扩增,大大提高PCR的扩增效 率,可以进行高灵敏度的RealTimePCR扩增反应。 另外,本制品中添加了TliRNaseH(耐热性RNaseH),以cDNA作为模板进行PCR反应时,可以最大限 度抑制由于cDNA中残存mRNA对PCR反应造成的阻害作用。
2×One Step RT-PCR Buffer Ⅲ TaKaRa Ex Taq HS(5 U/μl) PrimeScript RT Enzyme Mix Ⅱ PCR Forward Primer(10 μM) PCR Reverse Primer(10 μM) TaqMan® Probe Total RNA RNase Free dH2O Total 12.5 μl 0.5 μl 0.5 μl 0.5 μl 0.5 μl 1 μl*2 2 μl*3 7.5 μl 25 μl 1×...
Transfer the required amount of resin to a glass filter with a pore size of 10–20 µm. Apply a vacuum to the filter to remove excess ethanol. Add 5 bed volumes of deionized water to the resin and apply vacuum. Add 5 bed volumes of Equilibration Buffer to the ...
Fill the MED Probe with 0.1% polyethylenimine (PEI) in 25 mM borate buffer (pH 8.4). Leave it at room temperature overnight. (Make sure the electrodes are covered by the PEI.) Aspirate the PEI and rinse the MED Probe with double distilled water (DDW) at least 4 times. ...
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the precipitate was suspended in 20 μl of an elution buffer (50 mM NaH2PO4, 300 mM NaCl, 250 mM imidazole, pH 8.0). A supernatant was collected by centrifugation. The same elution procedure was repeated two more times. A total of 60 μl of a sample containing the PH1182 polypeptide...
Twenty-five micrograms of the chromosomal DNA obtained in Example 1 was partially digested with 1 unit of Sau3AI restriction enzyme in a buffer solution (50 mM Tris-HCl (pH 7.5), 10 mM MgCl2, 1 mM DTT, 100 mM NaCl), and agarose gel electrophoresis was carried out to recover 3-7 kb...