Tubes were rotated overnight at 4 °C, 2 mg of magnetic Dynabeads were added, and bead-antigen-antibody complexes were washed 3 times with CoIP lysis buffer and separated. Then, 1% SDS was added to beads in CoIP lysis buffer, boiled for 10 min, and Western blot performed for target ...
In addition, the present invention can be simply carried out by using a kit containing a cell-adhesive substance. The cell-adhesive substance to be contained in the kit may be in a form of solutions or lyophilized powders. The kit may contain a buffer for dissolving or diluting the cell-ad...
制品中含有Real Time PCR反应的适浓度TB Green,是一种2×浓度的Premix Type试剂,进行实验时,PCR反应液的配制十分方便简单。 本制品中还添加了Tli RNaseH(耐热性RNaseH),以cDNA作为模板进行PCR反应时,可以很好地抑制地由于cDNA中残存mRNA对PCR反应造成的阻害作用。 本制品Buffer经过改良,使反应特异性比TB ...
was added to a PCR buffer containing 50 ng of genomic DNA, 200 μM of a dNTP mixture, and 20 pmol of each of primers, to make up a total volume of 50 μl. For exons 10 to exon 19, each exon was individually subjected to amplification DNA reaction. The length mutation was ...
Buffer 10 l 1 TaKaRa Ex Taq HS 5 U l 0 4 l PrimeScript RT Enzyme Mix 0 4 l PCR Forward Primer 10 M 0 4 l 0 2 M 1 PCR Reverse Primer 10 M 0 4 l 0 2 M 1 TaqMan Probe 0 8 l 2 Total RNA 2 l 3 RNase Free dH2O 5 6 l Total 20 l 1 通常引物终浓度为 0 2 M 可以...
using our novel Capturem technology for efficient capture of antibody-bound target protein complexes. The included buffer set provides ease of use and rapid isolation of immunoprecipitated protein complexes, allowing faster downstream analysis. Each column can hold up to 850 µl of sample and ...
TaKaRa一步法RT-PCR提取法
digestion by controlling the time the sample is exposed to the protease. This kit contains Capturem Pepsin mini spin columns and an activation buffer. Each column can process 10–50 µg of sample in a loading volume of up to 850 µl, and requires a minimum elution volume of 200 µ...
制品中使用了抗 Taq 抗体的的 Hot Start 法用 DNA 聚合酶 TaKaRa Ex Taq HS ,与Real Time PCR 用最适 Buffer 组合,可以有效抑制非特异性的 PCR 扩增,大大提高 PCR 的扩增效率,可以 进行高灵敏度的 Real Time PCR 扩增反应。 另外,本制品中添加了 Tli RNaseH(耐热性 RNaseH) ,以cDNA 作为模板进行 PCR...
2.2. TaKaRa Ex Taq TaKaRa Ex Taq HSHS A 活性定义 以活性化的大马哈鱼精子 DNA 为模板/引物,在 74、30 分钟内使 10 nmol 总核苷酸掺 入酸不溶性沉淀物所需要的酶量定义为 1 个活性单位(unit)。 活性定义反应液: 25 mM TAPS buffer (pH9.3, 25) 50 mM KCl 2 mM MgCl2 0.1 mM DTT 200 M ...