These definitions, detergent solubility to define components of rafts, and sensitivity to cholesterol deprivation to define raft functions implicated sphingolipid- and cholesterol-rich lipid rafts in many cell functions. Despite extensive work, the basis for raft formation in cell membranes and the size...
such as isolated cellular membranes, can alleviate or entirely remove such biochemical challenges. Subsequently, we outline ssNMR experiments that involve the use of magic-angle-spinning and can be used to study membrane protein structure and their functional aspects. We pay specific attention to spect...
Mitochondrial dysfunction is one contributing factor, but its role at different stages of disease progression is not fully understood. Here, we showed that neural precursor cells and dopaminergic neurons derived from induced pluripotent stem cells (hiPSCs) from sPD patients exhibited a hypometabolism. ...
Fig. 1: Multiplexed imaging captures heterogenous HSV-1 gene expression between single cells. a, b HeLa cells were infected with HSV-1 multiplicity of infection (MOI) 0.3 and stained for UL29 (magenta) and UL19 (cyan) transcripts using smFISH at 1.5–12 hpi. Panel a, cells at 9 hpi....
The concept of liposomes was originally introduced by Bangham et al. in 1965. Today, it commonly refers to small closed vesicles with a bilayer wrapped in an aqueous structure formed by dispersing phospholipids and other lipids in water. Due to their structural similarity to biological membranes,...
Membranes were blocked with 5% skim milk, and then incubated for 1 h at room temperature with EflMsiA pAb (1:1000). After washing with TPBS, membranes were incubated with anti-rabbit-HRP (1:2000, GE Healthcare). Signals were detected using ECL Plus western blotting detection reagent (GE ...
PolyP has been detected in several subcellular compartments of mammalian cells, including the cytoplasm, plasma membranes, and organelles such as lysosomes, dense granules, mitochondria, and nuclei [2,3]. The major functions of polyP in mammalian cells include cell proliferation, angiogenesis, ...
From the very beginning, the challenge in the development of cryopreservation techniques was to cool down the biological material from room temperature to −196 °C, while ensuring the cellular function and integrity of cell organelles and membranes after warming to develop subsequent in vitro or ...
Sanitizers work by lysing cell membranes, blocking membrane functions (e.g. waste removal) and inactivating key enzymes. There are some combined cleaner/sanitizers but a sanitation step is still required. It is vital to make sure the surface is clean since sanitizers will preferentially bind to...
Exosome membranes contain cellular markers capable of targeting specific cell types. Exosomes are not cells. Thus, they are non-immunogenic, more stable, and easier to handle from a pharmaceutical delivery view. The contents of exosomes contain and deliver proteins, RNAs, DNAs and...