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Furthermore, the corresponding surface stiffness changes in the polymer film before (Figure 3e) and after being immersed in the 3′-sialyllactose solution (Figure 3f) were recorded using Young's modulus maps. The remarkable color change from purple (corresponding to a high modulus) to yellow (a...
The mean Young’s modulus of the polymer film decreased from 210 MPa to ~138 MPa. In comparison, there was no change in the surface morphology when the PEI thin film interacted with 3′-sialyllactose (Supplementary Figure 5). These data were consistent with the QCM-D dissipation changes...
Then, the enrichment efficiency of the PEI-g-DF-grafted microspheres for glycopeptides was evaluated using tryptic digests of bovine fetuin (a standard glycoprotein) mixed with different levels of BSA (a typical non-modified protein). Before the enrichment, the whole mass spectrum (MS) was occup...