For plastic parts to be painted electrostatically, a conductive primer needs to be applied first. In the case of SMC compression molded parts, in锕巓ld coating (IMC) is the primer material of choice, as it also
sgRNAs for CRISPR/Cas9 engineering, see Table S1 N/A N/A Primers for PCR screening, see Table S1 N/A N/A Primers for qPCR validation of ChIP-seq, see Table S1 N/A N/A Recombinant DNA Spen_SPOCmut_HV (Targeting R3532A R3534A point mutation to endogenous SPEN) Rodermund et al.,...
The short C9orf72 isoform was cloned from C9-L into pEGFP-C1 with the following 3' primer: tgacCTCGAGttacttgagaagaaagccttcatg. WDR41 and SMCR8 were also cloned into pcDNA3.1 myc his A (Invitrogen) to generate C-terminal myc his tagged con- structs. Additionally, C9orf72 and WDR41 ...
Quantitative PCR showed that each disruption mutant and ΔChr3 had a ~ 9.4-fold higher plasmid copy number than the wild type. Structural prediction models indicate that BSFP_068730/40 is structurally homologous to the structural maintenance of chromosomes (SMC) protein MukBE, which is respon...
In‐mold coating (IMC) is a thermosetting liquid applied to compression molded sheet molding compound (SMC) exterior automotive or truck body panels as an environmentally friendly primer to improve surface quality and make the part conductive for subsequent electrostatic painting. The IMC is injected ...
PCR primers for HTP genetic engineering, see Table S5 This paper N/A Software and Algorithms Bowtie2 v2.2.5 Langmead and Salzberg, 2012 http://bowtie-bio.sourceforge.net/bowtie2/index.shtml BLAST v2.3.0 NCBI ftp://ftp.ncbi.nlm.nih.gov/blast/executables/blast+/ Clustal Omega v1.2.0...
Spectinomycin-resistant transformants were screened on 100 μg/ml spectinomycin containing LB agar plates at 37°C followed by confirmation through colony PCR using gene-specific primers. Plasmid transformation efficiency Plasmid transformations were performed following the method from Doron et al. (2018...
(Bio-Rad). Differences in gene expression were calculated using the ΔΔCT method [61]. The housekeeping gene human RNA polymerase II (hRP) was used for internal normalization. Primers were purchased from Eurofins MWG Operon, Ebersberg, Germany. For primer sequences and TaqMan kits, please ...