1. The cytochemical staining of senescence associated β-galactosidase(SA-β-GAL)was applied to cultured neurons for 6、12 and 20 days. 方法:利用SD新生鼠进行海马神经元原代培养,分别取培养6、12和20d的神经元进行衰老相关β半乳糖苷酶(SA-β-GAL)的细胞化学染色。
Senescence-associated (beta)-galactosidase is widely used as a biomarker of replicative senescence. However, it remains unknown whether this is a distinct enzyme active at pH 6, and differentially expressed in senescence, or a manifestation of an increase in the classic acid lysosomal (beta)-galact...
The cytochemical staining of senescence associated β-galactosidase(SA-β-GAL)was applied to cultured neurons for 6、12 and 20 days. 方法:利用SD新生鼠进行海马神经元原代培养,分别取培养6、12和20d的神经元进行衰老相关β半乳糖苷酶(SA-β-GAL)的细胞化学染色。4...
Although the phenomenon of cellular senescence in human cells was first described several decades ago, major progress in understanding the mechanism and the role of cellular senescence started in the late 1990s, greatly facilitated by the discovery of the Senescence-Associated Beta-Galactosidase (SABG)...