2.Carlile TM, Martinez NM, Schaening C, et al. mRNA structure determines modification by pseudouridine synthase 1. Nat Chem Biol. 2019;15(10):966-974. 3.Bykhovskaya Y, Casas K, Mengesha E, Inbal A, Fischel-Gh...
Small RNAs guide these epigenetic modifications but how inherited small RNAs are distinguished in plants and animals is unknown. Pseudouridine (Ψ) is the most abundant RNA modification but has not been explored in small RNAs. Here, we develop assays to detect Ψ in short RNA sequences, ...
4. N. Guzzi, S. Muthukumar, M. Cieśla, G. Todisco, P. C. T. Ngoc, M. Madej, et al. Pseudouridine-modified tRNA fragments repress aberrant protein synthesis and predict leukaemic progression in myelodysplastic ...
Hendra C, et al. Detection of m6A from direct RNA sequencing using a multiple instance learning framework. Nat Methods. 2022 Dec;19(12):1590-1598. doi: 10.1038/s41592-022-01666-1. Epub 2022 Nov 10. Huang S, et al.Interferon inducible pseudouridine modification in human mRNA by quantitativ...
such nucleotide modification in mRNA translation (Kauffman et al., 2016;Thess et al., 2015), recent study confirmed thaturidinemodification and dsRNA impurity removal are the two most important factors determining the immunostimulating nature of synthesized mRNA (Nelson et al., 2020)....
, 1989). In addition, the presence of the poly(A) tail can also stimulate translation of the mRNA. Another covalent modification of mRNA that takes place in the nucleus is the methylation of some adenylate residues. Up to two methylated adenine have been found per mRNA of 1600 nucleotides....
RNA editing is the process by which ribonucleic acid (RNA) molecules are enzymatically modified post synthesis on specific nucleosides. Examples of RNA editing include pseudouridylation (the isomerization of uridine residues) and deamination (removal of an amine group from cytidine to give rise to ur...
In the Eukarya and Archaea, small RNA-guided pseudouridine modification is believed to be an essential step in ribosomal RNA maturation. While readily mode... DL Bernick,PP Dennis,M Hochsmann,... - 《Rna-a Publication of the Rna Society》 被引量: 32发表: 2012年 Constructive edge of urid...
[29] Mohanty BK, Kushner SR. The majority of Escherichia coli mRNAs undergo post-transcriptional modification in exponentially growing cells. Nucleic Acids Res 2006;34:5695–704. https://doi.org/10.1093/nar/gkl684. [30] Akichika S, Hirano S, Shichino Y, Suzuki T, Nishimasu H, Ishitani ...
methyladenosine (m6A), N1-methyladenosine (m1A), 5-methylcytosine (m5C), 5-hydroxymethylcytosine (hm5C), pseudouridine (Ψ), inosine (I), uridine (U) and ribose-methylation (2’-O-Me) [2,3,4] (Figs.1and2). m6A is the most abundant modification and was therefore thoroughly ...