Samples were checked for sonication efficiency using the criteria of 150–500 bp, by Agilent DNA bioanalyzer. ChIP-seq was carried out as previously described68 all liquid handling steps were performed on an Agilent Bravo NGS. Protein A Dynabeads (Invitrogen) were coupled with 2.5 µg of...
Samples were phenol/chloroform extracted and analyzed on a 6% polyacrylamide sequencing gel run at 1,500 V in 1X TBE, dried, and placed on x-ray film (Eastman Kodak, Rochester, NY) at -80°C with an intensifying screen. Electrophoretic mobility shift assay (EMSA) 32P-labeled double-...
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