C. Measurement of protein using bicinchoninic acid. Anal. Biochem. 150, 76-85 (1985). Stoscheck, C. M. Quantitation of protein. Meth. Enzymol. 182, 50-68 (1990).Tags MicrovolumeProtein Concentration DeterminationNanoDrop 2000c SpectrophotometerTraditional Spectrophot...
The NanoDrop 2000c Spectrophotometer - Microvolume Protein Concentration Determination (German)Basic ProtocolsJournal of Visualized Experiments
The Thermo Scientific NanoDrop 2000c Spectrophotometer solves this issue with an innovative sample retention system that holds microvolume samples between two measurement surfaces using the surface tension properties of liquids, enabling the quantification of samples in volumes as low as 0.5-2 μL. The...
to the ultrahigh mechanical stability: The ester bond locates at the force concentration point to lock the protein structure to a partially unfolded state with most of the native contacts preserved. On the other hand, the protein structure prevents the ester bond from water attack and makes this...
The data were analyzed using ASTRA software (v.6.1.2, Wyatt Technology) with a change in the refractive index with concentration, a dn/dc value, 0.185 ml g−1. Two-dimensional 1H-15N heteronuclear single quantum coherence measurement by nuclear magnetic resonance Two-dimensional (2D) ...
The density of the empty areas was set as background and was subtracted from each protein dot measurement. Bound preprotein to the translocase was plotted (y axis) against preprotein concentration used in the reaction (x axis). Hyperbolae were fitted by nonlinear regression, for one binding ...
Total protein in the soluble fraction was quantified by Nanodrop (1 mg = 1 A280) and standardized to the lowest protein concentration. Samples were mixed with protein loading dye (New England Biolabs, cat#B7703S), heated for 5 min at 95 °C, and 10 μl of each sample was loaded into...
In the retentostat phase, the measured product titer at a particular time point reflects the biomass concentration, qP, and initial titers, as well as wash-out dynamics. To estimate qPin the retentostat phase, a product mass balance was used (see Materials and Methods). Figure2F shows qPa...
Extracellular vesicles (EVs) are small nanometer-sized membrane sacs secreted into biological fluids by all cells. EVs encapsulate proteins, RNAs and metabolites from its origin cell and play important roles in intercellular communication events. Over th
These data were analyzed by the ASTRA software (Wyatt Technology), in which a change in the refractive index with concentration, a dn/dc value, 0.185 ml/g, was used. 2D 1H-15N HSQC measurement To confirm the core packing of designed proteins, we measured 2D 1H-15N HSQC spectra for ...