PROBLEM TO BE SOLVED: To provide a method for stably expressing a foreign DNA in chrysanthemum and to obtain chrysanthemum cells and a plant body of chrysanthemum in which the foreign DNA is stably expressed by the method.HAZAMA RYUTARO
631990 pLVX-EF1alpha-DsRed-Monomer-N1 Vector 10 ug * pLVX-EF1α-DsRed-Monomer-N1 is a lentiviral expression vector that can be used to generate high-titer lentivirus for transducing virtually any dividing or nondividing mammalian cell type, including primary and stem cells. The vector allow...
EF1α (EF1α)启动子是一种来源于延伸因子1α (Elongation factor 1 alpha, EF1α) 基因的强哺乳动物表达启动子,可在多种细胞中稳定驱动其下游基因的组成型表达,也可以用于干细胞、原代细胞、造血细胞等。 mCherry是一种来自于蘑菇珊瑚(Mushroom coral)的单体红色荧光蛋白,最大激发光(Excitation, Ex) 587nm/发...
PEF1,PEF1A,PEFLIN Promoter-Gluc启动子报告质粒 CDC42EP4 Promoter-Gluc启动子报告质粒 UBC7,E217K,UBE2G,UBE2G1 Promoter-Gluc启动子报告质粒 SEMG,SEMAG,SEMA5B,FLJ10372,KIAA1445 Promoter-Gluc启动子报告质粒 EXOSC4 Promoter-Gluc启动子报告质粒
EF1, elongation factor 1α; PGK, phosphoglycerate kinase 1; GFP, green fluorescent protein.B. Linking multiple genes with 2A is a better strategy than dual promoters for the expression of multiple genes. The expression of reprogramming factors OCT4, SOX2 and KLF4 was driven by the SFFV promoter...
several other constitutive RNApolII promoters, including human elongation factor-1 alpha (EF1α), human ubiquitin C (UBC), mouse phosphoglycerate kinase 1 (mPGK) and herpex simplex virus thymidine kinase (TK) promoters, which are the most commonly used promoters for gene expression in mammals. ...
The pTRIP-E3-Flag-TOP1 plasmid is derived from a pTRIP ΔU3 EF1α-H10-SUMO2 from J. Seeler (Institut Pasteur, Paris, France). Briefly, this construct contains the EF1α promoter (MluI-NsiI), and sequences coding for the Flag peptide (NsiI-BamHI), an optimized 93 amino acids Auxin...
The promoter of a soybean translation elongation factor EF1 alpha, a polypeptide that promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis, and fragments thereof and their use in promoting the expression of one or more heterologous nucleic acid...
94.The AAV particle of claim 88, wherein the promoter is cytomegalovirus (CMV) immediate-early enhancer and chicken beta-actin (CAG), cytomegalovirus (CMV), CMV/chicken β-actin (CMV/β-actin), elongation factor 1α (EF1α), phosphoglycerate kinase, ubiquitin C (UbC), CB, CBA, and CBh...
The EF1 protein also binds to an erythroid-specific promoter element of the mouse alpha-globin gene and to two sites within the chicken beta A-globin enhancer. These six EF1-binding sites are related by the consensus sequence A/TGATAA/GG/C. A minor protein of molecular weight 72,000 which...