Why The Primary Sequence Of Amino Acids In Any Polypeptide/Protein Is Fundamental To The Correct Functioning Of That Polypeptide/ProteinStephanie Reynolds
We purified a neutrophil chemotactic factor from a culture fluid of the PHA-activated human T-cell leukemia SKW-3 cells. The factor showed a 16-kDa basic protein by Tricin-SDS-polyacrylamide gel electorophoresis and analysis of amino acid composition. The primary amino acid sequence revealed that...
CCR3 facilitated infection by a more restricted subset of primary viruses, and binding of the CCR3 ligand, eotaxin, inhibited infection by these isolates. Utilization of CCR3 and CCR5 on the target cell depended upon the sequence of the third variable (V3) region of the HIV-1 gp120 ...
The amino acid sequence of sheep kappa A-casein. II. Sequence studies concerning the kappa A-caseinoglycopeptide and establishment of the complete primary ... The primary structure of sheep κ A-caseinoglycopeptide, the C-terminal portion of fκ A-casein released by chymosin, was established....
Other methods rely on an ensemble of functional data, such as genome-wide measures of co-expression and co-localization, which is often not available for non-model organisms. Although many homologous proteins may evolve to become highly dissimilar at the primary sequence level, they often retain ...
The primary structure of a protein consists of a sequence of amino acids and is a key factor in determining how a protein folds and functions. However, conventional methods for sequencing proteins, such as mass spectrometry and Edman degradation, suffer from short reads and lack sensitivity, so ...
A cDNA encoding the complete amino acid sequence of aminoacylase 1 (-acylamino acid aminohydrolase, ACY-1) [EC 3.5.1.14], a dimeric metalloprotein having two Znin the molecule, which catalyzes the deacylation of -acylated L-amino acids except L-aspartic acid, has been isolated from porcine ...
bovinespongi-formencephalopathy(BSE,alsoknownas“madcowdis-ease”)incattleandCreutzfeldt-Jakobdisease(CJD)inhu-mans.Allpriondiseasesarecurrentlyuntreatableandarealwaysfatal[4].SinceeachproteinbeginsasapolypeptidetranslatedfromasequenceofmRNAasalinearchainofaminoacids,itisinterestingtostudythefoldingratesofproteins...
The deduced amino acid sequences were verified independently to a large extent by direct peptide microsequencing of 66 and 84% of native Endo F2 and Endo F3, respectively. Structurally, the Endo F2 and Endo F3 genes code for a typically long leader sequence of 45 and 39 amino acids, ...
cDNA sequences encompassing the full coding region for the human muscle acetylcholine receptor (AChR) and γ subunits have been isolated. The deduced amino-acid sequences indicate that the mature subunit contains 473 amino acids and is preceded by a 20-amino-acid signal peptide. As predicted from...