3160724Certificate of Analysis2025年1月31日764551KT 3160686Certificate of Analysis2025年1月31日764551KT 2791363Certificate of Analysis2025年1月29日764551KT 显示5 条结果, 在上面搜索特定证书 申请COA 安全数据表 SDS Product Information Long Protocol: USB Poly (A) Tail-Length Assay Kit Protocol订购...
The Poly(A) Tail-Length Assay Kit allows you to quickly measure the poly(A) tails of multiple mRNAs in only three hours. This kit utilizes a novel G/I extension technique that preserves the 3' end of RNAs and ensures accurate measurement of poly(A) tail-length. And all reaction ...
Poly(A) tail length assayAdenylationYeast poly(A) polymeraseCapillary electrophoresismRNA polyadenylation functions in nuclear export, translation, and stability. We describe an efficient protocol designed to assess poly(A) tail length that is based on 3' tailing by yeast poly(A) polymerase and ...
The assay also provides quantitative estimates of the RNAs poly(A) tail length (1). RNA manipulations up to and including cDNA synthesis are carried out in a single tube, minimizing handling and possible RNase contamination. Most importantly, once the PAT cDNAs are synthesized they are stable ...
Using a polysome-based in vitro mRNA decay assay, we found that addition of recombinant HuD protein to the system increased the half-life of full-length, capped, and polyadenylated GAP-43 mRNA and that this effect was caused in part by a decrease in the rate of deadenylation of the ...
circadian deadenylase Nocturnin Shihoko Kojima1,2, Kerry L. Gendreau2, Elaine L. Sher-Chen1, Peng Gao1 & Carla B. Green1 mRNA poly(A) tails are important for mRNA stability and translation, and enzymes that regulate the poly(A) tail length significantly impact protein ...
While poly(A) tail length influences mRNA translation efficiency during the oocyte-to-embryo transition (OET), molecular mechanisms regulating maternal RNA poly(A) tail length are not fully understood. In this study, we identified MARTRE, a previously uncharacterized protein family (MARTRE1-MARTRE6...
The mammalian early embryo development requires translation of maternal mRNA inherited from the oocyte. While poly(A) tail length influences mRNA translation efficiency during the oocyte-to-embryo transition (OET), molecular mechanisms regulating materna
Widespread use of poly(A) tail length control to accentuate expression of the yeast transcriptome Control of poly( A) tail length can affect translation and stability of eukaryotic mRNAs. Although well established for individual cases, it was not known ... TH Beilharz,T Preiss - RNA 被引量:...
The poly(A) tail lengths were estimated after decoding hidden states with the model using Viterbi algorithm (Figures 1B and S1A, see “State decoding” and “Poly[A] length call”). Note that A118 was used as the longest spike-in because we failed to synthesize longer oligonucleotides of...