s5、制备cap/pei/poly(i:c)/sio2-s-ab:将共价功能化后的cap/pei/poly(i:c)/sio2颗粒加入至活化后的抗体中,所述抗体包括cd146、f4/80、igg1、igg2中的一种,3～5℃下反应20～28小时,离心分离得到cap/pei/poly(i:c)/sio2-s-ab纳米颗粒,将cap/pei/poly(i:c)/sio2-s-ab纳米颗粒溶解于超纯水中。
然后以胰腺癌细胞PANC-1为模型,研究其诱导PANC-1细胞分泌IFN-β的机制;通过AnnexinⅤ/PI染色检测胞质核酸诱导PANC-1细胞的凋亡情况,并阐明作用机制。结果 :通过筛选发现5ˊppp-ds RNA、poly(I:C)和poly(d A:d T)能够诱导多种肿瘤细胞产生IFN-β。poly(I:C)和poly(dA:dT)刺激PANC-1细胞后,信号通路中PKR...
技术方案:本发明提供了一种新抗原rna及免疫佐剂poly(i:c)复合疫苗,该复合疫苗包括peg-pll-plleu自组装纳米胶束,以及负载于peg-pll-plleu自组装纳米胶束上的新抗原rna疫苗和免疫佐剂poly(i:c);其中,所述新抗原rna其核苷酸序列如seqidno.1所示。 优选地,peg-pll-plleu自组装纳米胶束和新抗原rna疫苗的摩尔比在1...
Morphological changes and proliferation of microglia in response to LPS or poly(I:C). Microglia were treated with 100 ng/ml LPS, 10 µg/ml poly(I:C), or 3 µg/ml poly(I:C) for 24 h. (a) Representative images of double immunofluorescence labeling for GFAP (red) and Iba1 (green...
The full-length complementary (c)DNA of the Mx gene was cloned from the spleen of the tiger grouper, Epinephelus fuscoguttatus, injected with poly I:C by reverse-transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) methods. The 2163-bp cDNA contained an...
1 首先铺设路面，这里我们选择的是水平平直路面，拱形路面我们尝试后，发现无法支撑货车通过，所以我们采用的是水平平直路面。2 依靠下方的固定着力点，利用三角形结构，先固定中间3个路面连接点。3 继续利用三角形结构，固定左侧路面节点。4 右侧采用和左侧相同的结构，这里我们可以直接用鼠标右键框选左侧结构，复制后...
Pentamidine was used as a model drug in this study. Pentamidine/polyvinyl alcohol (PVA) hydrogel was prepared by freeze-thaw technique. Pentamidine loaded hydrogel was later microencapsulated in poly(lactide-co-glycolide) (PLGA) using solvent evaporation technique. The microcapsules were evaluated for ...
5. The composition of any one of claims 1 to 3, wherein said reactive component is polyethylene glycol monoacrylate, polyethylene glycol diacrylate or methoxypolyethylene glycol 550 monomethacrylate. 6. The composition of any one of ...
Loss of poly(ADP-ribose) polymerase-1 causes increased tumour latency in p53-deficient mice PARP-1-deficient mice display a severe defect in the base excision repair pathway leading to radiosensitivity and genomic instability. They are protected a... C.,Conde - 《Embo Journal》 被引量: 200发...
Their ability to swell arises from polyelectrolyte interactions and molecular structure of the gel network. A brief review of theories that relate the molecular structure of gels to their properties is given in the first part of this chapter. The main properties of interest are swelling volumes ...