Bohnert, H.U. et al. A putative polyketide synthase peptide synthetase fromMagnaporthe griseasignals pathogen attack to resistant rice.Plant Cell16, 2499–2513 (2004). ArticleGoogle Scholar Gaffoor, I. et al. Functional analysis of the polyketide synthase genes in the filamentous fungusGibberella ...
Genome mining of polyketide synthase (PKS)-nonribosomal peptide synthetase (NRPS) hybrid enzymes independent of trans-acting enoyl reductase using our in-house fungal draft genomic library led to the discovery of a unique PKS-NRPS hybrid enzyme, PbpsA, in a Pestalotiopsis fungus. Heterologously ...
ANALYZE HYBRID NRPS/PKS SEQUENCES 可进入 化合物快速分析界面,通过序列比对分析未知氨基酸 序列。点击 NRPSDB、PKSDB、ITERDB 和 CHSDB 链 接,分别进入相应子数据库界面[18]。 NRPSDB NRPSDB 数据库搜集了 17 个 NRP 簇和 5 个杂合 · 282 ·
BlastP alignment illustrated that the EpiA showed 53% identity to hybrid PKS−NRPS synthetase BuaA in A. burnettii, followed by the fusaridione A synthetase FsdS, fusarisetin A syn- thetase Fsa1, equisetin synthetase EqxS, pyrrolocin syn- thetase PrlS, pyrichalasin H synthase PyiS, ...
TeA is thought to be a hybrid of an isoleucine and two acetates12. Due to its tetramic acid-containing structure (Fig. 1a), TeA is also expected to be a product of a polyketide synthase and non-ribosomal peptide synthetase (PKS–NRPS) hybrid enzyme13. Typical fungal secondary metabolite ...
Polyketides such as the antibiotic erythromycin or the immunosuppressant rapamycin, and non-ribosomal peptides, such as the antibiotics penicillin or vancomycin, are important classes of natural products. The core of these molecules are biosynthesized by large polyketide synthases (PKS) and non-ribosomal...
A group or set of enzymes comprising such a NRPS domain and PKS enzymes may be referred to as a hybrid NRPS-PKS enzyme system or enzyme complex. The group of proteins and polypeptides encoded by the gene cluster as a whole are collectively referred to as the biosynthetic machinery for the ...
To establish the genetic bases for the biosynthesis of the mutagenic compound NG-391, we identified a 19,818聽kb genomic region harboring the predicted hybrid polyketide synthase-nonribosomal peptide synthetase NGS1, plus five additional ORFs. NGS1 knockouts generated by Agrobacterium-mediated ...
chains are recognized and processed will aid in the making of polyketide and peptide combinatorial libraries.; Yersiniabactin (Ybt), the tetracyclic iron chelating siderophore produced as a virulence factor in infections by the plague bacterium , is encoded by the hybrid NRP/PK/NRP Ybt synthetase....
An Aspergillus flavus secondary metabolic gene cluster containing a hybrid PKS–NRPS is necessary for synthesis of the 2-pyridones, leporins[J] . Jeffrey W. Cary,Valdet Uka,Zheng Han,Dieter Buyst,Pamela Y. Harris-Coward,Kenneth C. Ehrlich,Qijian Wei,Deepak Bhatnagar,Patrick F. Dowd,Stacey L...