The mechanisms underpinning the protective action of PFT-α against Meth have not been identified, and hence, their investigation is the focus of this study. Primary dopaminergic neuronal cultures were prepared from rat embryonic ventral mesencephalic tissue. High-dose Meth challenge reduced tyrosine ...
Pifithrin alpha (PFTalpha) is a chemical compound isolated for its ability to suppress p53-mediated transactivation. It can protect cells from p53-mediated apoptosis induced by various stimuli and reduce sensitivity of mice to gamma radiation. Identification of molecular targets of PFTalpha is likely...
PFT pretreatment also attenuated p53/Bax-mediated myocyte apoptosis and reduced leukocyte infiltration into the ischemic heart at 24 hours of reperfusion, compared to saline. These results suggest that both myocyte necrosis and apoptosis are associated with inflammation during MI/R. Furthermore, ...
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In this study, our aim was to evaluate the antiproliferative activity of these compounds in human hepatoma Hep G2 cells and examine the effects of pifithrin-alpha (PFT??; a specific p53 inhibitor) on their apoptotic signaling transduction mechanism. The antiproliferative activity was measured by ...
PFT alpha enhanced anticancer effect of TPT on cells was found mainly by two ways. Firstly, it increased the TPT accumulation in cells and nucleus and promoted the inhibition of TPT on activity of Topo I, and induced more DNA damage. Secondly, PFT alpha decreased formation of p53/mdm2 ...
PFT-alpha (O) treatment also decreased phospho-p53 positive neurons in the cortical contusion region. Our data suggest that PET-alpha (O) provided a significant reduction of cortical cell death and protected neurons from glutamate-induced excitotoxicity in vitro, as well as improved neurological ...
We found that PFTalpha, in addition to p53, can suppress heat shock and glucocorticoid receptor signaling but has no effect on nuclear factor-kappaB signaling. PFTalpha reduces activation of heat shock transcription factor (HSF1) and increases cell sensitivity to heat. Moreover, it reduces ...
In in vitro experiments hypothalamic slices containing supraoptic nuclei and intact pituitary were incubated with or without PFT. In all experiments, we observed accumulation of vasopressin (VP) in the cell perikarya after PFT injections; however, expression of VP mRNA was not changed. Analysis of ...
In order to verify the role of p53-associated pathway in ultraviolet (UV) induced apoptosis in human CS-B cells, the expression of p53 and p53 responsive genes was analysed in UV irradiated human cells after treatment with pifithrin-alpha (PFTalpha). PTFalpha effectively inhibited the induction...