Our study demonstrated that the protein expression pattern of the transcription factor pCREB/CREB, together with the protein expression of PGC-1 alpha, NRF1 and TFAM are all significantly reduced in early ages of 3xTg-AD mice. We also found reduced mRNA expression levels of PKAC-alpha, CREB,...
NAD+, decreased ADP and AMP contents and the ratio of AMP/ATP, reduced the level of ROS and MDA, enhanced SOD activity, evidently increased the levels of AMPK, SIRT1 and PGC-1 alpha mRNA, up-regulated the expressions of AMPK, pAMPK, SIRT1, PGC-1alpha, NRF1, TFAM and FOXO1 proteins...
Our data indicate that exposure to ropivacaine leads to reduced expression of the major mitochondrial regulator PGC-1 alpha and its downstream transcription factors NRF1 and TFAM. Ropivacaine treatment induces impairment of mitochondrial biogenesis by reducing mitochondrial mass, the ratio of mtDNA to n...
of Akt and ERK via activating Akt/Sirt1/PGC-1 alpha and MAPK/ERK signaling pathway cascades in L6 cells, and promote the mitochondrial biogenesis of L6 cells, thereby upregulating the mRNA expression of NDUFA9, SDHA, UQCRC2, ATP5a, Nrf1, Tfam and Sirt3 as well as down-regulating p53. ...