穿梭载体(sbuttle vector)指在两种宿主生物体内复制的载体分子,因而可以运载目的基因(穿梭往返两种生物之间)。穿梭质粒含原核和真核生物 2 个复制子,以确保两类细胞中都能扩增。 科淼生物的各种产品发货前经经过严格的多重验证,收到质粒后请短暂离心,加入20ul ddH2O溶解质粒,取2ul转化至对应感受态中,挑取单克隆重新...
0/0 收藏人数: 2 评论次数: 0 文档热度: 文档分类: 高等教育--生物学 系统标签: pet图谱说明书vectorubajixcmi Novagen•ORDERING800-526-7319•TECHNICALSUPPORT800-207-0144lacI(764-1843)ori(3277)Ap(4038-4895)f1origin(5027-5482)Bpu1102I(80)AvaI(158)XhoI(158)NotI(166)EagI(166)HindIII(173)Sal...
The pET-22b(+) vector carries an N-terminal pelB signal sequence for potential periplasmic localization, plus optional C-terminal His?Tag? sequence. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed...
pET-22b(+) Vector TB038 12/98 The pET-22b(+) vector (Cat. No. 69744-3) carries an N-terminal pelB signal sequence for potential periplasmic localization, plus optional C-terminal HisTag sequence. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR...
The pET-22b(+) vector (Cat. No. 69744-3) carries an N-terminal pelB signal sequence for potential periplasmic localization, plus optional C-terminal His•Tag® sequence. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 ...
它的全称是Pseudomonas putida expression vector,中文名称为假单胞菌表达载体。PET22b质粒在基因工程领域中具有很高的知名度,为科研人员提供了便利。 【PET22b质粒的组成与特点】 PET22b质粒主要由三个部分组成:启动子、目的基因插入区和选择性标记基因。启动子位于质粒的首端,负责启动目的基因的表达;目的基因插入区...
The pET-22b(+) vector carries an N-terminal pelB signal sequence for potential periplasmic localization, plus optional C-terminal His?Tag? sequence. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed...
The pET-22b(+) vector (Cat. No. 69744-3) carries an N-terminal pelB signal sequence for potential periplasmic localization, plus optional C-terminal His•Tag ®sequence. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 ...
pET22b(+) Vector Information 载体名称: 质粒类型: 表达水平: 启动子: 克隆方法: 克隆位点: 载体大小: 5' 测序引物及序列: 3' 测序引物及序列: 载体标签: 载体抗性: 筛选标记: 产品目录号: 稳定性: 组成型/诱导型: 病毒/非病毒: 克隆菌株: pET22b(+) 大肠杆菌蛋白表达载体 低拷贝 T7 promoter 多克隆...
备注:pET22b载体含有PelB信号肽序列, 能够将表达的目的蛋白定位在细胞外周质腔。 载体介绍 The pET-22b(+) vector carries an N-terminal pelB signal sequence for potential periplasmic localization, plus optional C-terminal His•Tag® sequence. Unique sites are shown on the circle map. Note that the...