综上所述，PD-L1的泛素化、磷酸化、糖基化之间相互调节，共同参与PD-L1的翻译后修饰过程。六、肿瘤微环境诱导PD-L1的表达肿瘤微环境中过度分泌的IFN-γ、TNF-α、白细胞介素等促炎细胞因子可以诱导肿瘤细胞中PD-L1的表达，促进肿瘤细胞的免疫逃逸。IFN-γ主要通过JAK/STAT1/ IRF途径在肿瘤中发挥作用[33]。TNF...
11. Chapoval AI, Ni J, Lau JS,et al.B7-H3: a costimulatory molecule for T cell activation and IFN-gamma production. Nat Immunol. 2001 Mar;2(3):269-74. 12. Philips GK, Atkins M.Therapeutic uses of anti-PD-1 and anti...
促进效应 CD8+T 细胞的细胞毒性功能:通过检测效应 CD8+T 细胞中干扰素 -γ(Interferon-gamma,IFN-γ)和颗粒酶 B(Granzyme B,GZMB)的表达,发现联合治疗显著提高了这些细胞毒性分子的表达水平,增强了效应 CD8+T 细胞的细胞毒性功能,使其能够更有效地杀伤肿瘤细胞 。 影响PD-1 表达:联合治疗导致肿瘤浸润 CD8+T ...
ThiemA, HesbacherS, KneitzH, et al.IFN-gamma-induced PD-L1 expression in melanoma depends on p53 expression[J].J Exp Clin Cancer Res, 2019, 38(1): 397.DOI:10.1186/s13046-019-1403-9. CortezMA, IvanC, ValdecanasD, et al.PDL1 regulati...
IFN-γ: Interferon-gamma IFN-α: Interferon-alpha IFN-β: Interferon-beta FBS: Fetal Bovine Serum FFPE: Formalin-fixed paraffin-embedded H&E: Hematoxylin and Eosin BSA: Bovine Serum Albumin IFNGR1: Interferon-gamma receptor 1 TPS: Tumor Proportion Score PDX: Patient-derived xenograf...
More importantly, IFN-gamma, but not IFN-alpha or IFN-beta stimulated neutrophils, acquired the capacity to suppress lymphocyte proliferation through the expression of programmed death ligand 1 (PD-L1). These data demonstrate that IFN-gamma-induced expression of PD-L1 on neutrophils enables ...
[29]. This effect is mediated through the IFN-gamma-Janus kinase (JAK) signaling pathway and transcriptional activator (STAT) pathway, with JAK2, located on chromosome 9p, playing a key role in regulating PD-L1 production. Mutations or amplifications within the JAK family can further increase ...
IFN-γ is the most effective inducer of PD-L1 expression, mainly derived from T cells or NK cells [21]. INF-γ Can interact with the INF-γ Receptor on the cell surface activates the expression of PD-L1 (Fig. 1). The PD-L1 protein will locate to the cell membrane and bind to ...
在有pd1/pd-l1拮抗剂存在时,ifn-gamma分泌的能力获得了增强。 树突状细胞的产生:从血沉棕黄层(buffycoats)(researchbloodcomponents,波士顿,马萨诸塞州)纯化获得pbmc。血沉棕黄层用pbs按照1:3稀释,于50ml管中加入超过15ml淋巴细胞分离液层,2000rpm离心25分钟。从梯度界面收集pbmc,用pbs洗3次。pbmc以约1-2×106...
(TCR) signaling. Upon ligating its receptor, PD-L1 has been reported to decrease TCR-mediated proliferation and cytokine production. PD-L1 expression was found to be abundant on many murine and human cancers and could be further up-regulated upon IFN-gamma stimulation. Thus, PD-L1 might play...