标准PCR试剂和试剂盒 2×Flash PCR MasterMix (Dye) 货号(SKU)包装规格是否现货价格数量 F665667-5ml 5ml期货 原价:¥297.90 +- F665667-40ml 40ml期货 原价:¥1,947.90 +- 大包装询价添加到收藏夹比较SDS中文版DATASHEET Skip to the end of the images gallery ...
Total RNA was isolated from cells by using the Qiagen RNeasy kit. cDNA was prepared from 500 ng total RNA by using the high capacity cDNA reverse transcription kit (Applied Biosystems) following manufacturers’ instructions. Q-PCR was performed by using Fast SYBR green PCR MasterMix (Applied Bi...
AccuStart II GelTrack PCR ToughMix (2X)2X mix containing optimized concentrations of MgCl 2, dNTPs, reaction buffer, hot-start DNA polymerase, stabilizers and gel loading dyes. GelTrack Loading Dye (50X)50X concentrated mixture of RT-PCR compatible, blue and yellow electrophoresis-tracking dyes. ...
普通PCR /2× SsoTaq PCR Mix/2× SsoTaq Gre...电议上海墨芊生物科技有限公司2022-06-30询价 PCR Mix染料(PCR Mix Dye)￥190.0/盒中国/上...VIP9年上海泽叶生物科技有限公司2025-03-27询价 PCR仪,朗基PCR仪A100电议杭州广州飞迪生物科技有限公司2021-10-20询价 ...
2. Reaction setup: The PCRreaction mix is assembled, which includes the DNA template, primers, DNA polymerase, and other necessary reagents. 3. Thermal cycling: The reaction mix is placed in a thermal cycler, which undergoes a series of temperature cycles. The cycles typically consist of the...
5、ln)、磁珠(rna binding beads)、洗涤液1(wash soln 1)、洗涤液2(wash soln 2) 荧光pcr检测试剂盒组分:2rt-pcr反应液(2rt-pcr buffer)、25rt-pcr酶混合液(25rt-pcr enzyme mix)、高g/c含量引物/探针检测增强剂(detection enhancer)、无核酸降解酶的水(nuclease-free water) 无水乙醇 无水异丙醇 2...
6、A. Principle of cDNA PreparationReverse TranscriptionProcedures of cDNA PreparationAssemble the reaction appropriate in a 0.25 ml PCR tube including:RNA templateNonspecific primerReverse transcriptaseDNA polymeraseBuffer reagentMix gently, spin briefly.Incubate in the thermacycler at:44C for 1 h92C ...
1.5 PCR 扩增及克隆测序 PCR 扩增反应体系(25 μL):病毒上下游引物 (10 μmol·L-1)各 1 μL,12.5 μL 2×Go Taq Green Master Mix,2 μL 模板,8.5 μL ddH2O.PCR 扩增反应 条件:94 ℃ 5 min;94 ℃ 30 s,X ℃(各病毒具体 退火温度见表 1)45 s,72 ℃ 1 min,35 个循环,最后 一轮循环...
SsoAdvanced™ Universal SYBR®Green Supermix (1725270) Hard-Shell®Low-Profile Thin-Wall 96-Well Skirted PCR Plates (HSP9601) Microseal®'B' Adhesive Seals (MSB1001) Configuration Options The CFX Connect Real-Time PCR Detection System can be configured with other PCR instruments so that it...
2.Q: How about your delivery time? A: Within 10 days after deposit, we always deliver the goods according to customer's requirement. 3.Q: What's about the minimum order quantity? A: The MOQ is 20GP, customer can mix different sizes in one 20GP. We suggest at least 40H...