and then lysed with RIPA buffer containing 1% PMSF on ice31. After centrifugation at 12000 g for 15 minutes, the supernatant was collected. The BCA reagent kit (Beyotime Biotechnology) was used to measure protein content. The cell extracts (50 μg protein) were loaded onto...
Many biochemical pathways produce reactive dicarbonyl intermediates, such as glyoxal and methylglyoxal (MG), which can further react with DNA, proteins, or other biomolecules to form AGEs[40]. In E. coli, it has been found that the predominant MG detoxification system consisted of glyoxalase ...
Many biochemical pathways produce reactive dicarbonyl intermediates, such as glyoxal and methylglyoxal (MG), which can further react with DNA, proteins, or other biomolecules to form AGEs[40]. In E. coli, it has been found that the predominant MG detoxification system consisted of glyoxalase ...
Hik36–Hik43 and Rre7 are known to form at TCS28, but a TCS pair consisting of Hik36–Hik43 and Rre6 has not been studied to date (Fig.1). To evaluate a potential physiological connection of Hik36–Hik43 and Rre6, individual mutants (Δhik36,Δhik43andΔrre6) were generated in...