we confirmed that PCC0208027 was mapped well in the ATP pocket of WT-HER2 (Fig.1(c)). The ethylene carbonyl group in PCC0208027 was flipped relative to its orientation in HER2/TAK-285 structures20. In the co-structure of PCC0208027 with HER2, the amino group in the quinazoline ring inte...
construct pPPD-BE–C, expressingtyrBfromE. coliandZ. mobilis tyrCin an operon, was not possible to generate in the WT background, but for PAL and TAL expressingSynechocystisstrains full segregation was successfully achieved. The construct pPPD-P–C-CM, generated to express PAA-...
We used a custom screening platform, based on 96-deepwell microplate culturing in an in house designed cultivation chamber integrated in a liquid handling robot for screening and selection; in addition we also used a more conventional system. The increased thermal tolerances of the isolated monoclona...
CP43 also has a role with D1 in binding the Mn4CaO5 cluster involved in water splitting (Ferreira et al., 2004). Assembly of PSII is thought to be a stepwise process (Komenda et al., 2004) proceeding through several intermediate complexes. Initially, the large pigment proteins form pre...
Download: Download full-size image Fig. 1. Arrangement of redox cofactors in the A and B branches of PSI. Chl1A and Chl1B together comprise the primary donor P700; Chl2A and Chl2B (A-1A/A-1B) are the Chl molecules closest to P700, and form the intermediate Chls A-1A and A-1B...
Many biochemical pathways produce reactive dicarbonyl intermediates, such as glyoxal and methylglyoxal (MG), which can further react with DNA, proteins, or other biomolecules to form AGEs[40]. In E. coli, it has been found that the predominant MG detoxification system consisted of glyoxalase ...
Full size image PCC 11801 could tolerate up to 15% CO2 level in the incubation chamber when grown in a shake flask (SF) (Fig. 2D). Surprisingly, we noticed that the growth of PCC 11801 was optimal at 0.5–1% CO2 in SF (Fig. 2D), unlike in MC (Fig. 2A,B). This may be becaus...
Many biochemical pathways produce reactive dicarbonyl intermediates, such as glyoxal and methylglyoxal (MG), which can further react with DNA, proteins, or other biomolecules to form AGEs[40]. In E. coli, it has been found that the predominant MG detoxification system consisted of glyoxalase ...
After the search from is defined the user can paste structures in MDL Molfile format using e.g. ISIS/Draw, MDL Draw, ChemDraw or any drawing package that can produce a Molfile. When the user selects a search form object that is associated with alphanumeric data, PCC displays a dialog ...
Abstract. When plants, algae, and cyanobacteria are exposed to excessive light, especially in combination with other environmental stress conditions such a