The accessibility of cell surface was increased in sap6-null strains According to the cell wall constitution, wall components could be released or extracted by chemical agents. We applied the β-mercaptoethanol (β-ME) to extract surface proteins of C. albicans strains because this is a simple ...
In the γC87K/γR242Q double mutant (Fig. 3E and F), hydrogen bonds between γC87K and βTPE381 and between γR242 and γE238 are no longer present; a new hydrogen bond is formed between γC87K and γR242Q. In this conformation, the ensemble-averaged distance between γC87K (...