0.运行Orthofinder 略1.建树进入到Single_Copy_Orthologue_Sequences文件夹中 ls *fa|while read file;do mafft --auto $file > $file.aln;done ls *aln|while read file;do seqkit sort $file|seqkit seq -w 0 > $file.format;done rm *.aln find *format | xargs paste -d " " > ../all.sing...