In his article published inNaturein 1970, Francis Crick stated the central dogma of molecular biology: information is passed from DNA (gene) to protein, through messenger RNA (mRNA).1Approximately 50 years later, the central dogma still stands. However, it has become just one among many mechan...
These were filtered to include only ORFs encoding peptides less than 100 amino acids and exclude those overlapping with larger proteins (Fig. 1c). Only those that meet the quality control metrics based on the parameters of known SEPs were retained (see methods). Of these, we filtered for ...
Asterisks indicate significant differences under two-sided paired Student’sttest (pvalue < 0.05).fSensitivity of detection of novel functional transcripts with PacBio, Illumina, or with the RACE enrichment followed by Nanopore sequencing. Note: the relatively short Illumina reads overlapping InSETes co...
Similarly, lncRNAs plasmacytoma variant translocation 1 (Pvt1) and RUNX1 overlapping RNA (RUNXOR) accelerated MDSC-mediated immunosuppression in LC in in vitro and in vivo models [77,78]. Conversely, other lncRNAs such as HOXA transcript antisense RNA myeloid-specific 1 (HOTAIRM1) and metastasis...
All SectionsBiomedical Engineering and MaterialsBiomedical Materials and NanomedicineCancer Biology and OncologyCell Biology and PathologyDrug Discovery and DevelopmentDrug Discovery, Development and DeliveryEndocrinology and Metabolism ResearchGene and Cell TherapyImmunology and ImmunotherapyMicrobiology in Human Health...
From the genetic point of view, the lncRNAs can be categorized as: (i) promoter-associated: non-coding transcripts deriving from the promoter region; (ii) sense oriented: lncRNAs and mRNAs are present on the same strand, with some overlapping exons; (iii) antisense oriented: lncRNAs and mRNAs...
(usually >10 kb);dexamples for various cases of lncRNAs overlapping with other genes on the same or the opposite strand;eenhancer RNAs expressed as uni- or bidirectional transcripts;fLncRNA transcribed from an intron of another gene;glncRNA hosting a miRNA. Noncoding genes are shown ingreen,...
Table S2. Lead eQTLs Overlapping the PIRs of Their Target Genes, Related to Figure 5 Table S3. Candidate Genes Prioritized on the Basis of GWAS and PCHi-C Data, Related to Figure 6 Data S1. Processed Datasets Generated in This StudyVolume...
Whether lincRNAs expressed with FPKM ≥ 1 or FPKM < 1, the minimum free energy of most overlapping lincRNAs were higher than unique lincRNAs (Figure3). Although the expressed abundances of the majority of overlapping lincRNAs were higher than unique lincRNAs in the cases of FPKM...
This unique feature is particularly interesting in the context of gene expression microarrays, where overlapping metagenes could identify genes that belong to multiple pathways or processes [4, 9]. Formal definition In this section we provide a mathematical formulation of the general NMF approach. ...