2)建立优化的冷冻方案(CryoStor CS10培养基+程序降温); 3)建立包括3D肿瘤球模型、NOD/SCID/IL2Rγnull(NSG)小鼠模型在内的多层次功能评价体系。所有实验均使用符合临床标准的脐带血来源细胞。 【GMP-compliant manufacturing】部分显示,采用G-rex生物反应器培养5周可实现平均767倍的扩增,获得1.4-2.4×109个高纯度(...
Chung YS, Son JK, Choi B, et al. Transplantation of human spleen into immunodeficient NOD/SCID IL2Rgamma(null) mice generates humanized mice that improve functional B cell development. Clin Immunol 2015;161:308-15.Chung YS, Son JK, Choi B, Park JB, Chang J, Kim SJ. Transplanta- tion...
SCID IL2rg skin transplantation humanized mice NOD/SCID NSG; 机译:SCID;IL2rg;皮肤移植;人源化小鼠;NOD / SCID;NSG; 入库时间 2022-08-21 11:28:51 相似文献 外文文献 中文文献 专利 1. Long-term maintenance of skin immune system in a NOD-Scid IL2r gamma(null) mouse model transplanted ...
IL2rgnull突变:Interleukin-2受体gamma链(IL-2Rγc,又称CD132)是具有重要免疫功能的细胞因子Il2、Il-4、Il-7、Il-9、Il-15和Il-21的共同受体亚基,该基因敲除后的小鼠机体免疫功能严重降低,通过多种受体阻断细胞因子信号传导,导致功能性NK细胞缺陷。 M-NSG严重免疫缺陷小鼠,缺失成熟T、B、NK细胞,可高效地植入...
4.2.4 The NOD/LtSz-scid with IL2γcnull (NSG) mouse NSG mouse strain is an immunodeficient mouse that knocks out the interleukin 2 receptor gamma chain (IL2Rγ) gene on the basis of NOD/SCID[77]. This mice strain lacks mature T and B lymphocytes and completely loses NK cell activ...
NOD-scid IL2R γnull mice engrafted with human PBMC reflect the immunological history of the donors and provide a complementary tool to in vitro studies. Thus, studies in this model might provide data with better translatability from bench to bedside....
The NOD.Cg-Prkdc scid Il2rg tm1Wjl /SzJ mouse strain, commonly known as NSG (for NOD SCID Gamma) is severely immunodeficient and thus is an excellent recip
Improved B cell development in humanized NOD-scid IL2Rgamma(null) mice transgenically expressing human stem cell factor, granulocyte-macrophage colony- stimulating factor and interleukin-3. Immun Inflamm Dis. 2016;4(4):427-40.Jangalwe S, Shultz LD, Mathew A, Brehm MA. Improved B cell ...
Human B cell development and antibody production in humanized NOD/SCID/IL-2Rgamma(null) (NSG) mice conditioned by busulfan. Journal of Clinical Immunology. 2011; 31:253-264. [PubMed: 20981478]Choi B, Chun E, Kim M, et al. Human B cell development and antibody production in humanized ...
However, high IL2 levels led to the development of acute GVHD. Moreover, NSG-(Kb Db)null (IAnull) mice injected with a PDX tumor and PBMC from an allogeneic donor supported significant tumor growth in the absence of acute GVHD. These data document that NSG mice deficient in murine MHC ...