Methods TNF was measured by flow cytometry and qPCR in neutrophils from 20 patients with RA before and after the start of TNF inhibitor therapy. Apoptosis was measured by morphology, and western blotting of pro- and antiapoptotic proteins in cell lysates. Nuclear factor 魏B (NF-魏B) activation...
2.7. Quantitative real-time polymerase chain reaction (qPCR) The adipose tissue was from inguinal fat. Total RNA was extracted from liver and adipose tissue using TRIzol reagent (Invitrogen). cDNA was prepared from 2 μg total RNA using a First Strand cDNA Synthesis kit (Thermo Fisher Scientific...
mRNA expression levels were quantified using RT-qPCR and normalized to housekeeping genes mRNA levels of β actin. Results depict significant alterations in the expression levels of NFκB, TLR-4, and SERCA2 among the study groups, highlighting potential molecular mechanisms underlying diabetes mellitus...
A reverse transcription product was used as template to conduct real-time polymerase chain reaction (PCR) using a StepOne Plus thermal cycler (Applied Biosystems, Foster City, CA) using 2x SYBR Green qPCR Master Mix (High ROX) (Bimake, Houston, TX) in accordance with the manufacturer’s ...
alteration, phagocytic activity change, and interleukin (IL) 6 and IL-17A production was examined. Moreover, the treated RAW 264.7 macrophages were analysed to predict signal transduction through next-generation sequencing (NGS) and the related gene expression was examined using real-time qPCR. ...
qPCR and immunohistochemistry results showed that Lu significantly reduced HMGB1 mRNA level and protein level. In addition, Lu significantly reduced the expression of HMGB1-TLR-NF-kappa B signaling pathway protein of intestine in mice. In conclusion, Lu significantly reduced and alleviated DSS-induced ...
RNA profiling of 39 pairs ESCC tumor and adjacent nontumor samples using RT-qPCR demonstrated that NKILA is significantly downregulated in ESCC tumor tissues, and NKILA expression levels were significantly decreased in advanced tumor tissues (III and IV) compared to early stages (I and II) (p <...
RT-qPCR was used to evaluate the expression level of RANKL-induced osteoclast-specific gene. Western blotting was conducted for analyzing potential mechanisms of inhibitory influence of Gly on the formation and function of osteoclasts in vitro. Ovariectomized (OVX) mice model, micro-CT and histo...
Furthermore, qPCR results displayed that Rb1 inhibited IL-1 beta, IL-6 and TNF-alpha production both in vivo and in vitro. The activation of Toll-like receptor 2 (TLR2) by S. aureus was inhibited by application of Rb1 as confirmed by results of immunofluorescence assay. The expression ...
qPCR and ELISA results showed that matrine dose-dependently reduced the expression of pro-inflammatory cytokines (TNF-alpha and IL-1 beta). To further elucidate the underlying mechanisms of this protective effect of matrine, LTA-stimulated bovine endometrial epithelial cells (bEECs) were employed in...