GFP-NF-kappaB chimeras were functional both in vitro and in vivo, as demonstrated by electrophoretic mobility shift assays and reporter gene studies. GFP-p65 was regulated by IkappaBalpha similar to wild type p65 and associated with its inhibitor even if both proteins were linked to a GFP ...
绿色荧光蛋白标记人细胞;MGC803-GFP 大鼠成年表皮角质形成层细胞完全培养基 100mLD-天冬酰胺,一水D-Asparagine monohydrate质量规格:0.99 L1210 小鼠细胞D-天冬氨酸D-Aspartic acid 质量规格:0.98 MMP8 Others Human 人 MMP-8 / CLG1 人细胞裂解液 (阳性对照) D-天冬氨酸4-叔丁酯D-Aspartic acid 4-tert-...
C-GFPSpark® RELA/NF-kappa B p65 cDNA 克隆背景 All these RELA/NF-kappa B p65 cDNA clone are full sequence confirmed. There are 30 RELA/NF-kappa B p65 expression cDNA clones with various fusion tags, especially GFPspark tag and OFPspark tag. RELA/NF-kappa B p65 expression cDNA clones...
A NF-kappaB reporter gene mouse (NF-kappaBEGFP) (EGFP, enhanced GFP) was crossed to the colitogenic susceptible strain IL-10-/- and derived into germfree conditions using embryo-transfer technology. Germfree IL-10wt/wt;NF-kappaBEGFP and IL-10-/-;NF-kappaBEGFP mice (wt, wild type) ...
www.nature.com/scientificreports OPEN Transcription factor NF-kappa B represses ANT1 transcription and leads to mitochondrial dysfunctions received: 11 October 2016 accepted: 13 February 2017 Published: 20 March 2017 Chen Zhang1, Hui Jiang2, Pin Wang3, Heng Liu3 & Xiulian Sun4 ...
Then, the clones were passaged into one well of a 24-well plate, and GFP-Cre Adenovirus was added into the well to cut the resistant gene. One allele disrupt clone were screened by PCR using proper primers. The second allele was then disrupted using the same method. 3. Results 3.1. ...
Results: GFP expression in ECA109 cells transfected with the E2F1 overexpression plasmid showed a needle-like shape. The proportion of pGV142-E2F1-transfected ECA109 cells in S-phase was elevated compared to the number of S-phase cells in the empty plasmid and negative control groups (P < ...
In addition, the subcellular localization of AjI kappa B alpha GFP fusion protein and the induction of AjI kappa B alpha alone or co-expression with Japanese eel IKK alpha (AjIKK alpha) in the activation of NF-kappa B, type I IFN and AP1 performed using Dual-Glo luciferase assay system ...