10 mM Tris-HCl 300 mM NaCl 1 mM DTT 0.1 mM EDTA 0.32 mM S-adenosylmethionine (SAM) 500 µg/ml BSA 50% Glycerol pH 7.4 @ 25°C 热失活 65°C for 20 minutes 甲基化敏感性 dam甲基化: Not Sensitive dcm甲基化: Not Sensitive
我们很高兴地宣布,所有反应缓冲液中均已不含 BSA(牛血清白蛋白)。从 2021 年 4 月开始,NEB 将含有 BSA 的反应缓冲液转换为含有重组白蛋白(rAlbumin)的缓冲液,用于限制性内切酶和某些 DNA 修饰酶。如需更多信息,请访问www.neb.cn/BSA-free。 同裂酶| 单字母标示| Pronunciation: ...
使用不含 BSA 的缓冲液时,建议补充 BSA。 在特定操作步骤建议的温度下温育。 * 参阅特定操作步骤以确定推荐的 dNTP 浓度。 参阅 Elevated temperatures, excessive amounts of enzyme, failure to supplement with dNTPs or long reaction times may result in recessed ends due to the 3´→ 5´ exonuclease...
BsaI, Restriction endonuclease Bsa I BsaI 快速限制性内切酶 替代NEB R3733V NEB R3733S NEB R3733L Effect of Digestion Fig.1 Add 1 μg plasmid and 1 μl BsaI (1-10 lane was diluted by 2 times gradient, 1 lane as stock solution) in 30 min at 37°C in a total reaction volume of ...
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 74°C. 贮存溶液 20 mM Tris-HCl 100 mM KCl 1 mM DTT 0.1 mM EDTA 200 µg/ml BSA 50% Glycerol 1X stabilizers pH 7.4 @ 25°C 热失活 否 ...
Muscle sections of 10 μm were fixed in 2% paraformaldehyde (PFA), blocked in phosphate buffered saline (PBS) with 10% foetal calf serum (FCS; Gibco), 1% bovine serum albumin (BSA; Sigma) and 1% saponin (Sigma) for 1 h. Mouse monoclonal antibody SERCA1 ATPase (diluted 1:1000, MA...
我们很高兴宣布,我们即将完成把所有反应缓冲液转换为无 BSA(牛血清白蛋白)缓冲液。从 2021 年 4 月开始,NEB 将含有 BSA 的反应缓冲液转换为含有重组白蛋白(rAlbumin)的缓冲液,用于限制性内切酶和某些 DNA 修饰酶。网站上的所有信息均已根据此变化更新。如需了解更多信息,请访问www.neb.cn/BSA-free。
BsaI, Restriction endonuclease Bsa I BsaI 快速限制性内切酶 替代NEB R3733V NEB R3733S NEB R3733L GMP grade Effect of Digestion Fig.1 Add 1 μg plasmid and 1 μl BsaI (1-10 lane was diluted by 2 times gradient, 1 lane as stock solution) in 30 min at 37°C in a total reaction vo...