Main Single-cell analyses are providing unprecedented opportunities to analyze the complexity of biological systems1. However, they are restricted to providing a snapshot of cellular processes, lacking analysis of dynamic behavior inherent to cell function. Therefore, the development of technologies that ...
“Our paper ranked top three on Nature BME’s most visited articles of 2019 [...] and gained a great deal of attention from academic institutes, industry sectors and funding agencies. In light of these results, I was awarded Lush Prize (UK) [...] and Ingber Lab man...
2. Inter- and intratumor heterogeneity of breast tumors is a major factor contributing to recurrence and metastasis after chemotherapy, which often come with acquired resistance to the therapeutic agents used in initial treatment. This is true for the human epidermal ...
The prevalence of chitin and chitosan in the mechanically crucial core ofR. delemarcell walls prompted us to evaluate the impact of chitin inhibitors on this fungus. We applied nikkomycin Z (Fig.4a), the only UDP-GlcNAc analog known to block chitin synthesis in fungi51,52, toR. delemarcult...
Cells were resuspended in complete medium, containing growth factors, stem-cell inducing factors and 8 µg/ml polybrene and laid over wells covered with BME. The virus was added and cells were incubated in normal culture conditions. After 24 h of infection a layer of BME was put on ...
Cell we seeded at a density of 600 cells per well in the middle wells of six-well plates that were coated with 2% Cultrex TM BME (R&D Sys A1569601). Cells were incubated at 37 °C and 5% CO2 for 2 h to allow attachment. Subsequently, plates were exposed to 3 or 4 Gy of...
Code availability The analysis scripts for screening and scRNA-seq are available at https://github.com/ShalekLab/Mead_et_al_NatBME_2021.References Ordovas-Montanes, J. et al. Allergic inflammatory memory in human respiratory epithelial progenitor cells. Nature 560, 649–654 (2018). Article CAS...
BME allows the cells to self-organize into 3D structures and stimulates cell proliferation. However, the use of BME is limiting the clinical applications of the organoids. There is a need for alternative tissue-specific and clinically relevant culture substrates capable of supporting organoid ...
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