Monoclonal nanobody sequences and purification.Keiji ItohSergei Y. Sokol
In this work, we introduce a new deep learning method to bypass these challenges by enabling the computational engineering of antibody and nanobody sequences indistinguishable from those obtained from immune systems. We call our method AbNatiV, as it provides an accurate quantification of the likelih...
Directly obtaining sequences for engineering Technical Route of Developing Nanobodies Nanobody development process includes alpaca immunization, phage library construction, nanobody screening, expression, purification, and validation stages. After alpaca immunization, B lymphocytes are isolated from the periphera...
Nanobodies have low immunogenicity as they have a small molecular weight and fewer epitopes available to bind to antigens. With a high sequence homology of >80% with human heavy-chain antibodies, nanobodies are suitable for engineering into humanized antibodies, which reduces the immunogenicity of ...
To validate the binding of the above nanobodies to HIV-1 CA, we generated nanobody fusion constructs (pLVX-puro-CANTDcb1-Fc, pLVX-puro-59H10-Fc, pLVX-puro-VHH9-Fc) composed of N-terminal secretion signal peptides, nanobody sequences, the human IgG Fc region (Capon et al., 1989) an...
The main data supporting the results of this study, including the nanobody sequences used, are available within the paper and itsSupplementary Information. The transcriptomic datasets generated during the current study are available from the corresponding authors upon reasonable request. ...
After removing duplicate sequences, the remaining sequences were synthesized and cloned into the pMECS vector. Nanobodies were expressed by E. coli and purified. All procedures were conducted in accordance with the "Guide for the Care and Use of Laboratory Animals" by the National Institutes of ...
et al. Constructing and purifying megabodies starting from individual nanobody sequences. Protoc. Exch. (2020) https://doi.org/10.21203/rs.3.pex-1033/v1. Geertsma, E. R. & Dutzler, R. A versatile and efficient high-throughput cloning tool for structural biology. Biochemistry 50, 3272–...
(CDR), make up the structure of a nanobody. Nanobodies’ CDR3 loop, which has a mean of 18 amino acid residues and a finger-like shape, allows them to bind antigens, and better antigen interaction results from longer CDR3 sequences [76]. In addition, due to their hydrophilic surface...
1.An aflatoxin M1 nanobody immunosorbent, comprising:a solid phase carrier; andan aflatoxin nanobody coupled to the solid phase carrier,wherein the aflatoxin nanobody consists of aflatoxin M1 nanobody 2014AFM-G2,wherein the aflatoxin M1 nanobody 2014AFM-G2 comprises the amino acid sequence of...