Our method relies on nanobody-based sensors, which enable proximity detection between subunits in time-resolved Förster resonance energy transfer (FRET) measurements. Additionally, given conformation-specific nanobodies, the activation of these complexes can be recorded in native brain tissue. Applied ...
Finally, these results do not exclude the possibility that other types of large assemblies or fibrils do penetrate into the cell cytosol, and would therefore be detected by the sensor. This issue could be tested more thoroughly in the future. It is known that many types of fibril-like assembl...
Microscopic clusters feature the composition of biochemical tetraspanin-assemblies and constitute building-blocks of tetraspanin enriched domains 2024, Scientific Reports The mesoscale organization of syntaxin 1A and SNAP25 is determined by SNARE-SNARE interactions 2021, eLife Light-guided intrabodies for on...
Here, we describe, to our knowledge, the first Nb-based inhibitor of a complement activation pathway. We have selected the nanobody hC3Nb1 against human C3b and show that it binds with low-nanomolar or picomolar affinity to native C3, C3b, iC3b, and the C3(H2O) mimic C3-methylamine (...
the many modular structural assemblies that make up the NPC have been broken down into further sub-assemblies which are then studied primarily by X-ray crystallography. Over the past decade, many of the structural elements have been characterized (reviewed in refs.2,19). We now have a complete...
A nanobody-based strategy for rapid and scalable purification of human protein complexes Introduction Epitope tags are employed in virtually any aspect of life sciences1,2. They are used in biotechnology to facilitate the expression and purification of recombinant proteins1or in cell biology to monito...
The major surface contact between P1.40 and the CHRD is an area of 850 Å2 (Protein Interfaces, Surfaces and Assemblies program [46]) that essentially involves Arg105, Thr109 and Phe110 (yellow sticks) of P1.40 and Phe456, Arg458, Arg476, Asp480, Glu481 and Leu528 (brown sticks) ...
Inference of macromolecular assemblies from crystalline state. J. Mol. Biol. 372, 774–797 (2007). Article CAS PubMed Google Scholar Roovers, R. C. et al. A biparatopic anti-EGFR nanobody efficiently inhibits solid tumour growth. Int. J. Cancer 129, 2013–2024 (2011). Article CAS Pub...
Another exciting possibility is that receptor activation in trans could operate across GPCR heteromers or even assemblies incorporating plasma membrane-localized proteins that are not GPCRs. Caution is needed as past work has shown that activation in trans is not possible for all GPCR-membrane protein...
Can nanobody-based drugs be successful in cancer treatment and diagnosis? Can anti-CTLA-4 nanobodies provide promising results in clinical research? What solutions are suggested to deal with the limitations of producing modified nanobodies with Fc-receptors?