The cyt P450 reductase transfers the first electron (1st e−) and second electrons (2nd e−) from the fully reduced FMN, respectively. While, cyt b5 transfers 2nd e− or both the 1st e− and 2nd e−. B, the reduction potentials of redox cofactors of the NADH-cyt b5 ET ...
Reduced nicotinamide adenine dinucleotide (NADH) is a fluorescent electron donor that binds to metabolic enzymes in the cytoplasm and mitochondria. The spectral properties of NADH and its phosphorylated form, NADPH, overlap, thus their combined fluorescence is denoted NAD(P)H. NADH has a vital role...
Correspondence to Richard H. Ebright or Bryce E. Nickels. Ethics declarations Competing interests The authors declare no competing financial interests. Extended data figures and tables Extended Data Figure 1 De novo transcription initiation by ATP and NCINs. a, Structures of ATP, NAD+, NADH, and...
(B–H) Analysis of cells as in (A). (B) SA-β-Gal staining: percent positive cells ± SD is shown in lower corner; scale bar, 250 μm. (C) WB for the indicated proteins. (D) NAD+/NADH ratio. (E) Total NADPH. (F) Ratio of GSH/GSSG. Individual levels of NAD, NADH, NADP...
1f)), mainly due to increased background in the minus ADPRC controls. Extensive overlap was detected between the two fragmented libraries and the unfragmented one (83.8% and 76.0%, respectively; Fig. 1e). Five out of the 12 genes explicitly reported in the previous study8 (Supplementary ...
E, expression of phospho-LKB1 and phospho-AMPK in the hearts of non-transgenic (N.Tg) and Sirt3 transgenic (SIRT3.Tg) mice. F and G, quantification of P-LKB1 and P-AMPK levels in the hearts of non-transgenic and Sirt3 transgenic mice. H, hemagglutinin-tagged SIRT3 was ...