Using the reaction with ADH, we examined the formation of NGDH and NHDH from NGD and NHD, respectively. Analyses using Orbitrap revealed that NAD, NGD, and NHD peaks decreased after the reaction with ADH. Furthermore, peaks corresponding to NADH, NGDH, and NHDH could be detected after ...
Reduced nicotinamide adenine dinucleotide (NADH) is a fluorescent electron donor that binds to metabolic enzymes in the cytoplasm and mitochondria. The spectral properties of NADH and its phosphorylated form, NADPH, overlap, thus their combined fluorescence is denoted NAD(P)H. NADH has a vital role...
F (= 0.999) is the fraction of deuterium in the solvent, and EV/K and EV are isotope effects minus 1 on kcat/Km and kcat, respectively. [S] and [E] are molar concentrations of substrate and enzyme, respectively. 3. Results 3.1. Kinetic comparison of wild-type AKR2B5 and Y51A ...
1f)), mainly due to increased background in the minus ADPRC controls. Extensive overlap was detected between the two fragmented libraries and the unfragmented one (83.8% and 76.0%, respectively; Fig. 1e). Five out of the 12 genes explicitly reported in the previous study8 (Supplementary ...