BBMVs isolated from rabbit renal outer cortex were preincubated in a buffer containing CdCl2 (50 microM in most cases) for 60 min at 37 degrees C and then tested for Pi uptake at 25 degrees C. CdCl2 treatment resulted in a marked attenuation of Na+-dependent Pi uptake with no changes ...
MW956 AppearanceLyophilized Powder Purity99% SolubilitySoluble in DMSO. Dilute with buffer, pH 7.5. Also soluble in buffer, pH 7.5. Storage Temp-20°C Additional Information MDL NumberMFCD08061890 InChIInChI=1S/C41H80O16P2.3Na/c1-3-5-7-9-11-13-15-17-19-21-23-25-27-29-34(42)53-31-...
Cells were pelleted at 5000 × g for 10 min at 4 °C, resuspended in 4 ml of lysis buffer (50 mm Tris-HCl, pH 8, 120 mmNaCl, 0.5% Igepal (Sigma CA-630), 5 mm dithiothreitol, 1 mm EDTA, 1 mm phenylmethylsulfonyl fluoride, 1 μg/ml leupeptin, 2 mg/ml lysozyme), and ...
Gentamicin causes endocytosis of Na/Pi cotransporter protein - Sorribas, Halaihel, et al. - 2001 () Citation Context ...d in a 6:4 mixture of cacodylate buffer (pH 7.4, containing 3 mM MgCl2 and adjusted to 300 mosmol with sucrose) and 10% hydroxyethyl starch in saline (HAES steril; ...
Here we used freshly isolated coronal mouse kidney slices and incubated them in a physiological buffer in the absence and presence of PTH with inhibitors and activators of the various signalling cascades to further study the events leading to internalization of NaPi-IIa. No alterations in the ...
The non-invasive sampling and the unrestricted avail- ability of this biological material make the analysis of saliva and levels of its components diagnostically significant.Aim of the study: To discuss factors affecting the rate of salivary flow, methods of sampling, saliva pH and buffer capacity...
Levi. Gentamicin causes endocytosis of Na/Pi cotransporter protein (NaPi-2). Kid. Int. 59: 1024-1036, 2001.Gentamicin causes endocytosis of Na/Pi cotransporter protein - Sorribas, Halaihel, et al. - 2001 () Citation Context ...d in a 6:4 mixture of cacodylate buffer (pH 7.4, ...