LPS-induced bioluminescence and AGP acute phase protein expression in female hCRP-Luc mice was mediated by IL-6. (a) Female hCRP-Luc mice were intraperitoneally injected with sterile PBS, LPS (100 ng/mouse), or IL-6 (1 μg/mouse). The liver bioluminescence was determined by IVIS at 22...
Briefly, 8 calibrated and logarithmically spaced von Frey monofilaments (bending forces: 0.008, 0.02, 0.04, 0.07, 0.16, 0.4, 0.6 and 1 g; Stoelting, Wood Dale, IL) were used. These were applied tangentially to the plantar surface for ~3–4 s with enough force to cause a slight ...
The concentrations of MCP-1 and IL-6 in the tissue culture supernatant were measured using a BD™ Cytometric Bead Array Flex Set. 2.5 Endothelial monolayer permeability and protein secretion hCMEC/D3 cells were plated in Vascular Permeability Assay kit plates (24-well) (Millipore Sigma) at 50...
Candidate genes identified using the expression-QTL strategy include: ATP-binding cassette, sub-family G (WHITE), member 1 (Abcg1), formyl peptide receptor 1 (Fpr1), gamma-aminobutyric acid (GABA) B receptor, 1 (Gabbr1); histocompatibility 2 genes: class II antigen E beta (H2-Eb1), ...
Deposition of amyloid beta (Aβ) and hyperphosphorylated tau along with glial cell-mediated neuroinflammation are prominent pathogenic hallmarks of Alzheimer’s disease (AD). In recent years, impairment of autophagy has been identified as anoth
The instrument we used in this study was an EASY nLC 1200 coupled with QE HF-X system (Thermo Fisher Scientific). We further validated our brain-unique DEPs results on an additional timsTOF flex (Bruker) mass spectrometry platform. The results showed that both mass spectrometry detected 6671 PG...
IL-17, and GM-CSF in splenic CD4+T cells or by microglia transcription and function. Rather,B. fragilissuppressed the production of GM-CSF by splenic CD4+T cells in APP/PS1 mice, suggesting reduced pro-inflammatory stimulation from T cells. Of note, APP/PS1-PBS mice had higher GM-CSF ...
Almost all effective treatments for non-alcoholic fatty liver disease (NAFLD) involve reduction of adiposity, which suggests the metabolic axis between liver and adipose tissue is essential to NAFLD development. Since excessive dietary sugar intake may b
9. The common mediators between aging and AD are predominantly pro-inflammatory and involve the increased expression of various cytokines, including interleukin-1-beta converting enzyme (IL-1β), interleukin (IL)-6, IL-18 and tumour necrosis factor alpha (TNF-α). Dysregulation of the ...
To express additional SCN1B or FGF12, cDNAs (Origene #RC209565, #RC215868) were cloned into an AAV-expression vector downstream of the hSyn promotor. A non-targeting AAV-Flex-GFP vector (from Dr. Il Hwan Kim) was used as a negative control. Cultured neurons were treated with these AAV...