A perfusion airlift bioreactor system that consists of the bioreactor, a media reservoir and a molecular weight cutoff (MWCO) filter positioned between the media reservoir and the airlift bioreactor.Ashot PetrossianAndy DeGiovanniUSUS4806484 * 1987年8月7日 1989年2月21日 Igb Products, Ltd. ...
Molecular weight cut off determination of UF membranes Techniques for the characterisation of ultrafiltration (UF) membranes generally use marker compounds of a known molecular weight (for example, dextrans and proteins) to challenge the membrane under controlled conditions of temperature, flow rate, and...
The coexpressed RdRp complex was incubated with additional nsp7 and nsp8 from the bacterial expression in a 1:1:2 molar ratio and incubated at 4 °C for 4 h. Incubated RdRp complex was concentrated with a 100 kDa molecular weight cut-off centrifugal filter unit (Millipore Corporation...
(Sigma Aldrich), and bound protein was eluted with 10 column volumes of 25 mM Na-HEPES pH 7.5, 150 mM NaCl, 500 mM imidazole. The eluate was concentrated to 500 µL using a 10 kDa molecular-weight cut-off Vivaspin filtration device (Sartorius), and further purified on a ...
Sucrose density gradient zones of interest were concentrated on Amicon Ultra centrifugal filter devices (Merck Millipore) with assigned molecular weight cut-off 10 kDa and applied to a pre-packed gel filtration column Superdex 200 10/300 GL (GE Healthcare). Gel filtration was carried out in Trici...
Three TaBiP genes exhibited significantly down-regulated expression following high molecular weight-glutenin subunit (HMW-GS) silencing. Drought stress induced significantly up-regulated expression of TaBiPs in wheat roots, leaves, and developing grains. Conclusions The high conservation of BiP sequences ...
Lyme disease in the United States is caused primarily by B. burgdorferi sensu stricto while other species are also prevalent in Europe. Genetic techniques have identified several chromosomal and plasmid-borne regulatory and virulence factors involved in
(Clontech) and the flow-through was collected to remove protease, cleaved His-tag, and uncleaved protein. The protein sample was incubated with excessive Nb39 (KOR/Nb39 m/m: 1:2) for 3 h. ĸOR-nalfurafine-Nb39 complexes were then applied to a 100 kDa molecular weight cut-off ...
(customer-made) on the column to remove the C-terminal 2MBP-tag in the buffer above at 4 °C. The flow-through was collected the next day and concentrated with a 100 kDa molecular weight cut-off concentrator (Millipore). The concentrated product was loaded onto a Superdex 200 ...
Structure elucidation of unknown small molecules by mass spectrometry is a challenge despite advances in instrumentation. The first crucial step is to obtain correct elemental compositions. In order to automatically constrain the thousands of possible ca