cDNA was synthesized from 2 μg total RNA with the Revert AidTM H Minus first strand cDNA synthesis kit (Fermentas, Hanover, MD, USA). SYBR green chemistry was used for quantitative determination of the mRNAs for RUNX-2, BMP-2, Type 1 collagen, Osterix, OCN, BMP-7 and GAPDH ...