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Here, we developed MoNaLISA, for Molecular Nanoscale Live Imaging with Sectioning Ability, a nanoscope capable of imaging structures at a scale of 45–65 nm within the entire cell volume at low light intensities (W-kW cm−2). Our approach, based on reversibly switchable fluorescent ...
coli strain DH10B (Invitrogen, Carlsbad, CA). Purified E6-AT protein was expressed in E. coli strain BL21 (NEB, Beverly, MA). Details of reagents, cloning, and protein expression experiments can be found in Table S1 and Supporting Notes 1 and 2. Preparation of Bacterial Films Individual ...
(Dojindo, Kumamoto, Japan). Forty-eight hours after transfection, the supernatant was collected and then used to infect C2C12 cells in the presence of 8 μg mL−1polybrene (Sigma). Infected C2C12 cells were selected using 3 μg mL−1blasticidin (Invitrogen, Carlsbad, CA) for 3...
The resulting suspension was dispersed in 900 μl of serum-free culture medium [Dulbecco's modified Eagle's medium (DMEM) for HeLa cells or RPMI1640 for Jurkat cells; Invitrogen Corp., Carlsbad, CA, USA]. The suspension was added directly to adherent HeLa cells cultured in a 35-mm dish ...
Next-generation sequence analyses were conducted using the Ion Torrent Personal Genome Machine (PGM; Life Technologies Corp., Carlsbad, CA, USA) (Yergeau et al.2012; Bondici et al.2013). DNA was extracted from both the homogenized sponge liquid and the electrically retrieved microbes with the ...
NIH 3T3 and MDA-MB-231 cell lines were maintained in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% FBS and 1% antibiotic/antimycotic solution (Invitrogen, Carlsbad, CA) in a humidified 5% CO2atmosphere at 37 °C. Prior to AFM experiments, cells were re-plated onto 50 mm...
The biofilms were either stained by membrane stain FM4-64 (1 μM final concentration, Molecular Probes, Invitrogen, Carlsbad, CA, USA) or live-dead staining 1603 The ISME Journal Biofilm formed by nitrogen-fixing bacteria D Wang et al 1604 kit with SYTO9 and propidium iodide (PI) following...