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7 ml of Dulbecco's phosphate-buffered saline containing 10 mM MgCl2,2 mM EGTA, 0.15% BSA, 0.15 mM bacitracin, 1.0 mM phenylmethylsulfonyl fluoride (PMSF), and 100 kallikrein inhibitory units (KIU)/ml aprotonin, pH 7.0 at 22°C, using a Polytron (Brinkmann, setting 6.0) for 20 sec. ...
At this sampling rate (6.2 pixels/\(\mu m\)), the typical time for imaging a single tissue core (1 mm2 area) was approximately 12 sec. Figure 1 (a) The SLIM module added on to a commercial phase contrast microscope. (b) Four frames are acquired to compute one phase image by ...
DIA is hence becoming the strategy of choice for extensive profiling-based approaches such as SEC-MS30 and has recently been applied in high-throughput subcellular phosphoproteomics31. Here, we harness the power of DIA for generating label-free DOMs. We show that proteomic depth, precision and ...
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For measurements of real samples, an initial signal offset was adjusted by adding a highly-diluted pretreated sample with concentration (~5 ng/L). The error bars arose from the upper and lower limit of multi-measurements operated in a certain concentration. References 1. Lei, M., Tie, B...
17, 1980 Sec. 102(e) Date Mar. 17, 1980 PCT Filed Jul. 17, 1979 PCT Pub. No. WO80/00231 PCT Pub. Date Feb. 21, 1980.A die-cutting machine comprises two form-carrying rolls (10,12) operable in tim... BISHOP Thomas Desmond 被引量: 16发表: 1982年 Container label and system ...
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In the present study, we take advantage of the powerful precompensation scheme presented in the previous sec- tion to work with a much smaller (by a factor of 4.5) pure-silica inner core DC-PCF ensuring high spatial resolution without losing the desired temporal confinement of excitation pulses...
<div p-id="p-0001">Provided are methods of determining differences between nucleic acids in a test sample and a reference sample. In certain embodiments the methods are used for detecting and mapping